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Functional Screening of Intracardiac Cell Transplants Using Two-Photon Fluorescence Microscopy

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Abstract

Although the adult mammalian myocardium exhibits a limited ability to undergo regenerative growth, its intrinsic renewal rate is insufficient to compensate for myocyte loss during cardiac disease. Transplantation of donor cardiomyocytes or cardiomyogenic stem cells is considered a promising strategy for reconstitution of cardiac mass, provided the engrafted cells functionally integrate with host myocardium and actively contribute to its contractile force. The authors previously developed a two-photon fluorescence microscopy-based assay that allows in situ screening of donor cell function after intracardiac delivery of the cells. This report reviews the techniques of two-photon fluorescence microscopy and summarizes its application for quantifying the extent to which a variety of donor cell types stably and functionally couple with the recipient myocardium.

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Acknowledgments

This study was supported by NIH grant RO1HL075165 to Michael Rubart and the Riley Children’s Foundation.

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Authors and Affiliations

  1. Department of Pediatrics, Riley Heart Research Center, Indiana University School of Medicine, 1044 West Walnut Street, Indianapolis, IN, 46202, USA

    Wen Tao, Mark H. Soonpaa, Loren J. Field, Anthony B. Firulli, Weinian Shou & Michael Rubart

  2. Division of Cardiology, Department of Medicine, Krannert Institute of Cardiology, Indiana University School of Medicine, Indianapolis, IN, 46202, USA

    Mark H. Soonpaa, Loren J. Field & Peng-Sheng Chen

Authors
  1. Wen Tao
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  2. Mark H. Soonpaa
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  3. Loren J. Field
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  4. Peng-Sheng Chen
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  5. Anthony B. Firulli
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  6. Weinian Shou
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  7. Michael Rubart
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Correspondence to Michael Rubart.

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Tao, W., Soonpaa, M.H., Field, L.J. et al. Functional Screening of Intracardiac Cell Transplants Using Two-Photon Fluorescence Microscopy. Pediatr Cardiol 33, 929–937 (2012). https://doi.org/10.1007/s00246-012-0314-8

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  • DOI: https://doi.org/10.1007/s00246-012-0314-8

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