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Novel and label-free colorimetric detection of radon using AuNPs and lead(II)-induced GR5 DNAzyme-based amplification strategy

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Abstract

Radioactive radon decays into a stable daughter product, 210Pb, which was used as the detection target to determine the radon radiation dose in a new technique. Pb2+ triggers DNAzyme to cleave a molecular beacon (MB), resulting in the stem–loop structure opening and forming two single DNA strands (ssDNA). The ssDNA binds to unmodified gold nanoparticles and effectively prevents their aggregation in a salt solution. The detached enzyme strands continue to complement the remaining MB to amplify the response signal. The method proposed in this study exhibited a good linear relationship for Pb2+ and radon concentrations in the range of 6.22 × 102–1.02 × 105 Bq h/m3 with a detection limit of 186.48 Bq h/m3 using an ultraviolet–visible spectrometer. In practical applications, this sensitive method can avoid radioactive damage in field testing, and the detection limit meets the national standard in China. Importantly, this simple, highly sensitive strategy uses simple equipment and has a strong anti-interference ability.

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Funding

The authors received support from the National Natural Science Foundation of China (No. 81473021) and Graduate Student Research Innovation Project in Hunan Province (No. CX2017B557).

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Authors and Affiliations

  1. College of Public Health, University of South China, No. 28 Changsheng West Road, Hengyang, 421001, Hunan, China

    Hongwen Liu, Yating Chen, Chunli Song, Gang Tian, Shiya Li, Guiying Yang & Changyin Lv

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  1. Hongwen Liu
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  2. Yating Chen
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  3. Chunli Song
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  4. Gang Tian
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  5. Shiya Li
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  7. Changyin Lv
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Correspondence to Changyin Lv.

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Liu, H., Chen, Y., Song, C. et al. Novel and label-free colorimetric detection of radon using AuNPs and lead(II)-induced GR5 DNAzyme-based amplification strategy. Anal Bioanal Chem 410, 4227–4234 (2018). https://doi.org/10.1007/s00216-018-1077-8

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  • DOI: https://doi.org/10.1007/s00216-018-1077-8

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