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Extraction and detection of bisphenol A in human serum and urine by aptamer-functionalized magnetic nanoparticles

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Abstract

A new type of magnetic nanoparticles (MNPs), as the absorbents of bisphenol A (BPA), was prepared by functionalization of Fe3O4@SiO2 with BPA-specific aptamer in this work. ssDNA aptamer was immobilized on the Fe3O4@SiO2 surface through biotin-avidin interactions, playing a role of the specific probe for BPA. The resultant materials (Apt-MNPs) exhibited outstanding magnetic responsibility and can be separated efficiently by the magnetic field. Experimental results also showed that Apt-MNPs had large adsorption capacity and high competitive selectivity for the targeted compound BPA. Furthermore, Apt-MNPs were adopted as the specific absorbents to extract and enrich BPA from human serum and urine samples. Therefore, an efficient detection method of BPA was developed in combination with high-performance liquid chromatography (HPLC). The linearity of the method was over a range of 5–10,000 ng mL−1 with a correlation coefficient of 0.99997, and the limit of detections (LODs) for serum and urine were 2.0 and 1.0 ng mL−1, respectively. The recoveries of BPA in the spiked human serum and urine samples were 90.8 ± 7.3% (RSD) and 92.3 ± 1.5%, respectively. Our results demonstrated that Apt-MNPs were high-performance adsorbents for extracting and enriching BPA, resulting in fast and efficient detection of BPA in serum and urine samples.

Aptamer-MNPs were effective for BPA separation from serum and urine

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (21107083), Wenzhou Public Welfare Science and Technology Project (S20150019, Y20160024), Zhejiang Medical and Health Science & Technology Project (2018PY032), Health Bureau of Zhejiang Province (11-CX29), Zhejiang Province College Students Science and Technology Innovation Project (Xinmiao Talent Program) (2015R413065), and Research project for College Students of Wenzhou Medical University (wyx2016101096).

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Author notes

  1. Yu Su and Chenggang Shao contributed equally to this work.

Authors and Affiliations

  1. Center of Scientific Research, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China

    Yu Su, Chenggang Shao, Jinxia Qi, Renshan Ge & Zhenkun Lin

  2. The Department of Gastroenterology and Hepatology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 310018, China

    Xielin Huang & Huaqin Guan

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  1. Yu Su
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  2. Chenggang Shao
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Correspondence to Huaqin Guan or Zhenkun Lin.

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Human and animal rights and informed consent

Human urine sample used in this study was collected from a healthy volunteer. This work was performed with the written informed consent of the healthy volunteer. The studies were approved by The Ethics Committee of The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, and performed in accordance of the ethical standards.

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Su, Y., Shao, C., Huang, X. et al. Extraction and detection of bisphenol A in human serum and urine by aptamer-functionalized magnetic nanoparticles. Anal Bioanal Chem 410, 1885–1891 (2018). https://doi.org/10.1007/s00216-017-0801-0

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  • DOI: https://doi.org/10.1007/s00216-017-0801-0

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