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Fully automated focused infrared microashing combined with use of ICP-based instruments for rapid analysis of multiple elements in biological samples

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Abstract

A fully automated focused infrared microashing sample preparation system was proposed for preparation of biological samples with high organic matter content for the determination of multiple elements combined with inductively coupled plasma optical emission spectroscopy and inductively coupled plasma mass spectrometry. The whole ashing procedure, including sample transfer, carbonization and oxidation of the sample, dissolution of ash, constant volume control, and homogenization of the solution, was automatically controlled. Gold-plated infrared tubes were used to produce and focus infrared radiation to heat the sample. Ozone was used to accelerate the carbonization of samples at a lower temperature to avoid the production of large amounts of empyreumatic oil. In addition, the self-designed double-layer tube serves as a site for ashing and carbonization of the sample and as a container for dissolving ash, as well as for holding the solution. This is the only container in the entire system to reduce the risk of pollution. Eight biological certified reference materials were used as examples to evaluate the performance of the proposed device. A sample ashing pretreatment cycle, from solid sample to liquid solution, took only 40 min and simultaneously treated 12 samples. Except for individual results, the relative errors between the certified values and recorded values for 38 micro and trace elements, including Ca, Mg, Na, P, Li, Be, Sc, Ti, V, Mn, Co, Ni, Cu, Zn, Rb, Sr, Y, Mo, Ag, Cs, Ba, Tl, Th, U, and rare earth elements, were typically less than 30%. The relative standard deviations for five determinations were typically less than 15%.

Automated dry ashing sample preparation system. ICP inductively coupled plasma

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Funding

This study was funded by the National Instrumentation Program of China (grant no. 2013YQ510391), the Shaanxi Province Science and Technology Project (grant no. 2015GY054), the National Science Fund of China (grant no. 21575113), the Specialized Research Fund for the Doctoral Program of Higher Education (grant no. 20126101110013), and the Scientific Research Foundation of Shaanxi Provincial Key Laboratory (grant nos 15JS100 and 16JS099).

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Authors and Affiliations

  1. Institute of Analytical Science, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi’an, Shaanxi, 710069, China

    Ni Zhang & Jianbin Zheng

  2. Xi’an Northwest Geological Institute for Nonferrous Metals Co. Ltd., 25 Xiying Road, Xi’an, Shaanxi, 710054, China

    Ni Zhang

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  1. Ni Zhang
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  2. Jianbin Zheng
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Correspondence to Jianbin Zheng.

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The authors declare that they have no competing interests.

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This article does not contain any studies with human participants or animals performed by any of the authors. The human hair sample used in this work was a Chinese national certified reference standard material, which was purchased from a commercial institute in China.

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Zhang, N., Zheng, J. Fully automated focused infrared microashing combined with use of ICP-based instruments for rapid analysis of multiple elements in biological samples. Anal Bioanal Chem 410, 1489–1499 (2018). https://doi.org/10.1007/s00216-017-0790-z

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  • DOI: https://doi.org/10.1007/s00216-017-0790-z

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