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Selective extraction and determination of fluoroquinolones in bovine milk samples with montmorillonite magnetic molecularly imprinted polymers and capillary electrophoresis

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Abstract

A sensitive and selective method for separating fluoroquinolones (FQs) from bovine milk samples was successfully developed using montmorillonite magnetic molecularly imprinted polymers (MMMIPs) as adsorbents. MMMIPs were prepared using montmorillonite as carrier, fleroxacin (FLE) as template molecule, and Fe3O4 magnetite as magnetic component. MMMIPs possessed high adsorption capacity of 46.3 mg g−1 for FLE. A rapid and convenient magnetic solid-phase extraction procedure coupled with capillary electrophoresis was established with MMMIPs as adsorbents for simultaneous and selective extraction of four FQs in bovine milk samples. Limits of detection ranged between 12.9 and 18.8 μg L−1, and the RSDs were between 1.8 % and 8.6 %. The proposed method was successfully applied to spike bovine milk samples with recoveries of 92.7 %–108.6 %.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (21464006), the Major Projects of Department of Education of Guangdong Province (2014KZDXM074), the Project of Department of Education of Guangdong Province (2013KJCX0191 and 2014KTSCX193), the Project of Science and Technology Innovation Project of Zhaoqing City (2013 F013), the Special Projects of University Talent Introduction of Guangdong Province(2013197), the Science and Technology Planning Project of Zhaoqing High-Technology Zone (2012B01003002), and the Natural Science Foundation of Zhaoqing University (201318).

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Authors and Affiliations

  1. School of Chemistry & Chemical Engineering, Zhaoqing University, Zhaoqing, 526061, China

    Hongwu Wang, Yanqing Liu, Shoulian Wei, Su Yao & Huichang Huang

  2. Department of Chemistry, East China Jiaotong University, Nanchang, 330013, China

    Jiali Zhang

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  1. Hongwu Wang
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  2. Yanqing Liu
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Correspondence to Hongwu Wang or Shoulian Wei.

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Wang, H., Liu, Y., Wei, S. et al. Selective extraction and determination of fluoroquinolones in bovine milk samples with montmorillonite magnetic molecularly imprinted polymers and capillary electrophoresis. Anal Bioanal Chem 408, 589–598 (2016). https://doi.org/10.1007/s00216-015-9140-1

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