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Analysis of stem cell lipids by offline HPTLC-MALDI-TOF MS

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Abstract

MALDI-TOF MS is traditionally used for “proteomics”, but is also a useful tool for lipid analysis. Depending on the applied matrix, however, some lipid classes are more sensitively detected than other ones and this may even lead to suppression effects if complex mixtures are analyzed. Therefore, a previous separation into the individual lipid classes is necessary. Using artificial lipid mixtures or easily available tissue extracts, it has been already shown that HPTLC-(High Performance Thin-Layer Chromatography)-separated lipids can be conveniently analyzed by MALDI-TOF MS directly on the TLC plate. Here we present an initial TLC-MALDI study of the lipid composition of ovine mesenchymal stem cells. Due to the complex composition of these cells, data are also compared to lipids extracted from human erythrocytes. It will be shown that even very minor lipid classes can be easily detected and with much higher sensitivity than by common staining protocols. Additionally, MS images of the developed TLC plates will be shown and potential applications, new methods of data analysis as well as problems discussed.

 

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References

  1. Salgado AJ, Oliveira JT, Pedro AJ, Reis RL (2006) Curr Stem Cell Res Ther 1:345–364

    CAS  Google Scholar 

  2. Schiller J, Fuchs B, Arnold K (2006) Curr Org Chem 10:1771–1789

    Article  CAS  Google Scholar 

  3. Fuchs B, Schiller J, Cross MA (2007) Chem Phys Lipids 150:229–238

    Article  CAS  Google Scholar 

  4. Peterson BL, Cummings BS (2006) Biomed Chromatogr 20:227–243

    Article  CAS  Google Scholar 

  5. Watson AD (2006) J Lipid Res 47:2101–2111

    Article  CAS  Google Scholar 

  6. Wenk MR (2005) Nat Rev Drug Discov 4:594–610

    Article  CAS  Google Scholar 

  7. Pulfer M, Murphy RC (2003) Mass Spectrom Rev 22:332–364

    Article  CAS  Google Scholar 

  8. Lee SH, Williams MV, DuBois RN, Blair IA (2003) Rapid Commun Mass Spectrom 17:2168–2176

    Article  CAS  Google Scholar 

  9. Schiller J, Süß R, Arnhold J, Fuchs B, Leßig J, Müller M, Petković M, Spalteholz H, Zschörnig O, Arnold K (2004) Prog Lipid Res 43:449–488

    Article  CAS  Google Scholar 

  10. Schiller J, Süß R, Fuchs B, Müller M, Zschörnig O, Arnold K (2007) Front Biosci 12:2568–2579

    Article  CAS  Google Scholar 

  11. Hillenkamp F, Peter-Katalinić J (2007) MALDI MS - A Practical Guide to Instrumentation, Methods and Application. Wiley-VCH, Weinheim

    Google Scholar 

  12. Gellermann GP, Appel TR, Davies P, Diekmann S (2006) Biol Chem 387:1267–1274

    Article  CAS  Google Scholar 

  13. Petković M, Schiller J, Müller M, Benard S, Reichl S, Arnold K, Arnhold J (2001) Anal Biochem 289:202–216

    Article  Google Scholar 

  14. Stubiger G, Belgacem O (2007) Anal Chem 79:3206–3213

    Article  Google Scholar 

  15. Estrada R, Yappert MC (2004) J Mass Spectrom 39:412–422

    Article  CAS  Google Scholar 

  16. Schiller J, Süß R, Fuchs B, Müller M, Petković M, Zschörnig O, Waschipky H (2007) Eur Biophys J 36:517–527

    Article  CAS  Google Scholar 

  17. Touchstone JC (1995) J Chromatogr B Biomed Appl 671:169–195

    Article  CAS  Google Scholar 

  18. Schiller J, Süß R, Fuchs B, Müller M, Zschörnig O, Arnold K (2003) Chromatographia 57:297–302

    Article  Google Scholar 

  19. Fuchs B, Süß R, Nimptsch A, Schiller J (2008) Chromatographia, in press

  20. Nakamura K, Suzuki Y, Goto-Inoue N, Yoshida-Noro C, Suzuki A (2006) Anal Chem 78:5736–5743

    Article  CAS  Google Scholar 

  21. Dreisewerd K, Muthing J, Rohlfing A, Meisen I, Vukelic Z, Peter-Katalinić J, Hillenkamp F, Berkenkamp S (2005) Anal Chem 77:4098–4107

    Article  CAS  Google Scholar 

  22. Rohlfing A, Müthing J, Pohlentz G, Distler U, Peter-Katalinić J, Berkenkamp S, Dreisewerd K (2007) Anal Chem 79:5793–808

    Article  CAS  Google Scholar 

  23. Fuchs B, Schiller J, Süß R, Schürenberg M, Suckau D (2007) Anal Bioanal Chem 389:827–834

    Article  CAS  Google Scholar 

  24. Nouri-Sorkhabi MH, Wright LC, Sullivan DR, Kuchel PW (1996) Lipids 31:765–770

    Article  CAS  Google Scholar 

  25. Tannert A, Kurz A, Erlemann KR, Müller K, Herrmann A, Schiller J, Töpfer-Petersen E, Manjunath P, Müller P (2007) Eur Biophys J 36:461–475

    Article  CAS  Google Scholar 

  26. Schulz R, Zscharnack M, Hanisch I, Geiling M, Hepp P, Bader A (2008) Biomed Mater Eng 18:55–70

    Google Scholar 

  27. Folch J, Lees M, Stanley GHS (1957) J Biol Chem 226:497–509

    CAS  Google Scholar 

  28. Schiller J, Arnold K (2002) Med Sci Monit 8:MT205–MT222

    CAS  Google Scholar 

  29. Schiller J, Müller M, Fuchs B, Arnold K, Huster D (2007) Curr Anal Chem 3:283–301

    Article  CAS  Google Scholar 

  30. White T, Bursten S, Frederighi D, Lewis RA, Nudelman E (1998) Anal Biochem 10:109–117

    Article  Google Scholar 

  31. Suckau D, Resemann A, Schürenberg M, Hufnagel P, Franzen J, Holle A (2003) Anal Bioanal Chem 376:952–965

    Article  CAS  Google Scholar 

  32. Fuchs B, Schober C, Richter G, Süß R, Schiller J (2007) J Biochem Biophys Meth 70:689–692

    Article  CAS  Google Scholar 

  33. Puppato A, DuPré DB, Stolowich N, Yappert MC (2007) Chem Phys Lipids 150:176–185

    Article  CAS  Google Scholar 

  34. Petković M, Schiller J, Müller J, Müller M, Arnold K, Arnhold J (2001) Analyst 126:1042–1050

    Article  Google Scholar 

  35. Al-Saad KA, Siems WF, Hill HH, Zabrouskov V, Knowles NR (2003) J Am Soc Mass Spectrom 14:373–382

    Article  CAS  Google Scholar 

  36. Mehl JT, Gusev AI, Hercules DM (1997) Chromatographia 46:358–364

    Article  CAS  Google Scholar 

  37. Schiller J, Süß R, Petković M, Zschörnig O, Arnold K (2002) Anal Biochem 309:311–314

    Article  CAS  Google Scholar 

  38. Sugiyama E, Hara A, Uemura K, Taketomi T (1997) Glycobiology 7:719–724

    Article  CAS  Google Scholar 

  39. He H, Conrad CA, Nilsson CL, Ji Y, Schaub TM, Marshall AG, Emmett MR (2007) Anal Chem 79:8423–8430

    Article  CAS  Google Scholar 

  40. Schiller J, Müller K, Süß R, Arnhold J, Gey C, Herrmann A, Leßig J, Arnold K, Müller P (2003) Chem Phys Lipids 126:85–94

    Article  CAS  Google Scholar 

  41. Hidaka H, Hanyu N, Sugano M, Kawasaki K, Yamauchi K, Katsuyama T (2007) Ann Clin Lab Sci 37:213–221

    CAS  Google Scholar 

  42. Stoeckli M, Chaurand P, Hallahan DE, Caprioli RM (2001) Nature Med 7:493–496

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the German Research Council (DFG Schi 476/5–1) and the Federal Ministry of Education and Research (Grant BMBF 0313836).

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Authors and Affiliations

  1. Faculty of Medicine, Institute of Medical Physics and Biophysics, University of Leipzig, Härtelstrasse 16–18, 04107, Leipzig, Germany

    Beate Fuchs, Jürgen Schiller & Rosmarie Süß

  2. Center for Biotechnology and Biomedicine, Cell Techniques and Applied Stem Cell Biology, Deutscher Platz 5, 04103, Leipzig, Germany

    Matthias Zscharnack & Augustinus Bader

  3. Institute of Biology, Humboldt University, Invalidenstrasse 43, 10115, Berlin, Germany

    Peter Müller

  4. Bruker Daltonik GmbH, Fahrenheitstrasse 4, 28359, Bremen, Germany

    Martin Schürenberg, Michael Becker & Detlev Suckau

Authors
  1. Beate Fuchs
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  2. Jürgen Schiller
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  3. Rosmarie Süß
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  4. Matthias Zscharnack
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  5. Augustinus Bader
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  6. Peter Müller
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  7. Martin Schürenberg
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  8. Michael Becker
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  9. Detlev Suckau
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Corresponding author

Correspondence to Jürgen Schiller.

Additional information

Beate Fuchs, Jürgen Schiller and Rosmarie Süß contributed equally to this work.

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Fuchs, B., Schiller, J., Süß, R. et al. Analysis of stem cell lipids by offline HPTLC-MALDI-TOF MS. Anal Bioanal Chem 392, 849–860 (2008). https://doi.org/10.1007/s00216-008-2301-8

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  • DOI: https://doi.org/10.1007/s00216-008-2301-8

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