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Reversed-phase liquid chromatography and argentation chromatography of the minor capsaicinoids

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Abstract

An investigation of the liquid chromatography of the minor capsaicinoids in a commercial capsaicinoid mixture is reported. Twelve stationary phases including C8, C18, C30, phenyl, and cation-exchange chemistries were examined in combination with isocratic aqueous methanol and aqueous acetonitrile mobile phases. A phenyl stationary phase and aqueous acetonitrile mobile phase baseline-resolved 7 of 11 capsaicinoids, and selected ion chromatograms (LC–ESI-MS) demonstrated this was the most effective reversed-phase separation. Argentation chromatography with an alkyl or phenyl column and aqueous silver nitrate–methanol mobile phase revealed the presence of the 6-ene-8-methyl and 6-ene-9-methyl homocapsaicin isomers and the absence of 7-ene-9-methyl homocapsaicin. A mixed phenyl–cation-exchange stationary phase (charged with silver ion) enabled unique and useful separations of the capsaicinoids.

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Acknowledgements

The research was funded in part by the National Institute of Justice through the Office of Law Enforcement Standards of the National Institute of Standards and Technology (NIST). RQT thanks Oberlin College and its sabbatical leave program and the Analytical Chemistry Division of NIST for financial support and other support of the research.

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Authors and Affiliations

  1. Department of Chemistry, Oberlin College, 119 Woodland Street, Oberlin, OH, 44074, USA

    Robert Q. Thompson

  2. Chemical Science and Technology Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, Stop 8392, Gaithersburg, MD, 20899-8392, USA

    Karen W. Phinney, Lane C. Sander & Michael J. Welch

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  1. Robert Q. Thompson
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  2. Karen W. Phinney
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  3. Lane C. Sander
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Thompson, R.Q., Phinney, K.W., Sander, L.C. et al. Reversed-phase liquid chromatography and argentation chromatography of the minor capsaicinoids. Anal Bioanal Chem 381, 1432–1440 (2005). https://doi.org/10.1007/s00216-005-3098-3

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  • DOI: https://doi.org/10.1007/s00216-005-3098-3

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