Abstract
Rationale
Understanding the neurobiological mechanisms mediating dominance and competitive aggression is essential to understanding the development and treatment of various psychiatric disorders. Previous research suggests that these mechanisms are both sexually differentiated and influenced substantially by social experience. In numerous species, GABAA receptors in the lateral septum have been shown to play a significant role in aggression in males. However, very little is known about the role of these GABAA receptors in female aggression, the role of social experience on GABAA receptor–mediated aggression, or the roles of different GABAA subtypes in regulating aggression.
Objectives
Thus, in the following set of experiments, we determined the role of social experience in modulating GABAA receptor–induced aggression in both male and female Syrian hamsters, with a particular focus on the GABAA receptor subtype mediating these effects.
Results
Activation of GABAA receptors in the dorsal lateral septum increased aggression in both males and females. Social housing, however, significantly decreased the ability of GABAA receptor activation to induce aggression in males but not females. No significant differences were observed in the effects of GABAA receptor activation in dominant versus subordinate group-housed hamsters. Finally, examination of potential GABAA receptor subtype specificity revealed that social housing decreased the ratio of δ extrasynaptic to γ2 synaptic subunit GABAA receptor mRNA expression in the anterior dorsal lateral septum, while activation of δ extrasynaptic, but not γ2 synaptic, GABAA receptors in the dorsal lateral septum increased aggression.
Conclusions
These data suggest that social experience can have profound effects on the neuronal mechanisms mediating aggression, especially in males, and that δ extrasynaptic GABAA receptors may be an important therapeutic target in disorders characterized by high levels of aggression.
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Abbreviations
- GABAAR:
-
GABAA receptor
- dLS:
-
Dorsal lateral septum
- adLS:
-
Anterior dorsal lateral septum
- CDP:
-
Chlordiazepoxide
- THIP:
-
Gaboxadol
- MS:
-
Medial septum
References
Albers HE (2012) The regulation of social recognition, social communication and aggression: vasopressin in the social behavior neural network. Horm Behav 61(3):283–292. https://doi.org/10.1016/j.yhbeh.2011.10.007
Albers HE (2015) Species, sex and individual differences in the vasotocin/vasopressin system: relationship to neurochemical signaling in the social behavior neural network. Front Neuroendocrinol 36:49–71. https://doi.org/10.1016/j.yfrne.2014.07.001
Albers HE, Bamshad M (1998) Role of vasopressin and oxytocin in the control of social behavior in Syrian hamsters (Mesocricetus auratus). Prog Brain Res 119:395–408 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10074802
Albers HE, Walton JC, Gamble KL, McNeill JKT, Hummer DL (2017) The dynamics of GABA signaling: revelations from the circadian pacemaker in the suprachiasmatic nucleus. Front Neuroendocrinol 44:35–82. https://doi.org/10.1016/j.yfrne.2016.11.003
Alvarez DD, Giacomini D, Yang SM, Trinchero MF, Temprana SG, Buttner K A, ..., Schinder AF (2016). A disynaptic feedback network activated by experience promotes the integration of new granule cells. Science, 354(6311), 459–465. doi:https://doi.org/10.1126/science.aaf2156
Belelli D, Harrison NL, Maguire J, Macdonald RL, Walker MC, Cope DW (2009) Extrasynaptic GABAA receptors: form, pharmacology, and function. J Neurosci 29(41):12757–12763. https://doi.org/10.1523/JNEUROSCI.3340-09.2009
Brain PF (1972a) Effects of isolation on adrenocortical and gonadal function in male and female golden hamsters. J Endocrinol 53(3):62–63 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/5064673
Brain PF (1972b) Effects of isolation/grouping on endocrine function and fighting behavior in male and female golden hamsters. (Mesocricetus auratus Waterhouse). Behav Biol 7(3):349–357 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/5063900
Bredewold R, Schiavo JK, van der Hart M, Verreij M, Veenema AH (2015) Dynamic changes in extracellular release of GABA and glutamate in the lateral septum during social play behavior in juvenile rats: implications for sex-specific regulation of social play behavior. Neuroscience 307:117–127. https://doi.org/10.1016/j.neuroscience.2015.08.052
Brickley SG, Mody I (2012) Extrasynaptic GABA(A) receptors: their function in the CNS and implications for disease. Neuron 73(1):23–34. https://doi.org/10.1016/j.neuron.2011.12.012
Brown N, Kerby J, Bonnert TP, Whiting PJ, Wafford KA (2002) Pharmacological characterization of a novel cell line expressing human alpha(4)beta(3) delta GABA(A) receptors. Br J Pharmacol 136(7):965–974. https://doi.org/10.1038/sj.bjp.0704795
Chang CH, Hsiao YH, Chen YW, Yu YJ, Gean PW (2015) Social isolation-induced increase in NMDA receptors in the hippocampus exacerbates emotional dysregulation in mice. Hippocampus 25(4):474–485. https://doi.org/10.1002/hipo.22384
Dar MS (2001) Modulation of ethanol-induced motor incoordination by mouse striatal A(1) adenosinergic receptor. Brain Res Bull 55(4):513–520 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/11543952
DaVanzo JP, Chamberlain J, McConnaughey MM (1986) Influence of environment on GABA receptors in muricidal rats. Pharmacol Biochem Behav 25(1):95–98 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/3018798
De Vries GJ (2004) Minireview: sex differences in adult and developing brains: compensation, compensation, compensation. Endocrinology 145(3):1063–1068. https://doi.org/10.1210/en.2003-1504
Delville Y, De Vries GJ, Ferris CF (2000) Neural connections of the anterior hypothalamus and agonistic behavior in golden hamsters. Brain Behav Evol 55(2):53–76. https://doi.org/10.1159/000006642
Depaulis A, Vergnes M (1983) Induction of mouse-killing in the rat by intraventricular injection of a GABA-agonist. Physiol Behav 30(3):383–388 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/6683411
Depaulis A, Vergnes M (1985) Elicitation of conspecific attack or defense in the male rat by intraventricular injection of a GABA agonist or antagonist. Physiol Behav 35(3):447–453 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/2999844
Drickamer LC, Vandenbergh JG (1973) Predictors of social dominance in the adult female golden hamster (Mesocricetus auratus). Anim Behav 21(3):564–570 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/4795936
Drickamer LC, Vandenbergh JG, Colby DR (1973) Predictors of dominance in the male golden hamster (Mesocricetus auratus). Anim Behav 21(3):557–563 Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/4795935
Ehlen JC, Paul KN (2009) Regulation of light’s action in the mammalian circadian clock: role of the extrasynaptic GABAA receptor. Am J Phys Regul Integr Comp Phys 296(5):R1606–R1612. https://doi.org/10.1152/ajpregu.90878.2008
Farrant M, Nusser Z (2005) Variations on an inhibitory theme: phasic and tonic activation of GABA(A) receptors. Nat Rev Neurosci 6(3):215–229. https://doi.org/10.1038/nrn1625
Ferris CF, Delville Y, Irvin RW, Potegal M (1994) Septo-hypothalamic organization of a stereotyped behavior controlled by vasopressin in golden hamsters. Physiol Behav 55(4):755–759 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/8190806
Frye GD, McCown TJ, Breese GR (1983) Characterization of susceptibility to audiogenic seizures in ethanol-dependent rats after microinjection of gamma-aminobutyric acid (GABA) agonists into the inferior colliculus, substantia nigra or medial septum. J Pharmacol Exp Ther 227(3):663–670 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/6317842
Gray CL, Norvelle A, Larkin T, Huhman KL (2015) Dopamine in the nucleus accumbens modulates the memory of social defeat in Syrian hamsters (Mesocricetus auratus). Behav Brain Res 286:22–28. https://doi.org/10.1016/j.bbr.2015.02.030
Grimes JM, Ricci LA, Melloni RH Jr (2003) Glutamic acid decarboxylase (GAD65) immunoreactivity in brains of aggressive, adolescent anabolic steroid-treated hamsters. Horm Behav 44(3):271–280 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/14609549
Han S, Tai C, Westenbroek RE, Yu F H, Cheah CS, Potter GB, . . . Catterall WA (2012). Autistic-like behaviour in Scn1a+/- mice and rescue by enhanced GABA-mediated neurotransmission. Nature, 489(7416), 385–390. doi:https://doi.org/10.1038/nature11356
Hanchar HJ, Chutsrinopkun P, Meera P, Supavilai P, Sieghart W, Wallner M, Olsen RW (2006) Ethanol potently and competitively inhibits binding of the alcohol antagonist Ro15-4513 to alpha4/6beta3delta GABAA receptors. Proc Natl Acad Sci U S A 103(22):8546–8551. https://doi.org/10.1073/pnas.0509903103
Harmon AC, Moore TO, Huhman KL, Albers HE (2002) Social experience and social context alter the behavioral response to centrally administered oxytocin in female Syrian hamsters. Neuroscience 109(4):767–772 Retrieved from PM:11927158 http://ac.els-cdn.com/S0306452201005231/1-s2.0-S0306452201005231-main.pdf?_tid=058ecb1a-4522-11e6-9c31-00000aacb35d&acdnat=1467992490_5807aba66432699e58f29ae78c3df62f
Huhman KL, Solomon MB, Janicki M, Harmon AC, Lin SM, Israel JE, Jasnow AM (2003) Conditioned defeat in male and female Syrian hamsters. Horm Behav 44(3):293–299 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/14609551
Irvin RW, Szot P, Dorsa DM, Potegal M, Ferris CF (1990) Vasopressin in the septal area of the golden hamster controls scent marking and grooming. Physiol Behav 48(5):693–699 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/2082369
Lee G, Gammie SC (2009) GABA(A) receptor signaling in the lateral septum regulates maternal aggression in mice. Behav Neurosci 123(6):1169–1177. https://doi.org/10.1037/a0017535
Lee V, Maguire J (2014) The impact of tonic GABAA receptor-mediated inhibition on neuronal excitability varies across brain region and cell type. Front Neural Circuits 8:3. https://doi.org/10.3389/fncir.2014.00003
Leroy F, Park J, Asok A, Brann DH, Meira T, Boyle LM, ..., Siegelbaum SA (2018). A circuit from hippocampal CA2 to lateral septum disinhibits social aggression. Nature, 564(7735), 213–218. doi:https://doi.org/10.1038/s41586-018-0772-0
Locci A, Pinna G (2018) Social isolation as a promising animal model of PTSD comorbid suicide: neurosteroids and cannabinoids as possible treatment options. Prog Neuro-Psychopharmacol Biol Psychiatry 92:243–259. https://doi.org/10.1016/j.pnpbp.2018.12.014
Maguire J, Mody I (2008) GABA(A)R plasticity during pregnancy: relevance to postpartum depression. Neuron 59(2):207–213. https://doi.org/10.1016/j.neuron.2008.06.019
McCann KE, Sinkiewicz DM, Rosenhauer AM, Beach LQ, Huhman KL (2018) Transcriptomic analysis reveals sex-dependent expression patterns in the basolateral amygdala of dominant and subordinate animals after acute social conflict. Mol Neurobiol. https://doi.org/10.1007/s12035-018-1339-7
McDonald MM, Markham CM, Norvelle A, Albers HE, Huhman KL (2012) GABAA receptor activation in the lateral septum reduces the expression of conditioned defeat and increases aggression in Syrian hamsters. Brain Res 1439:27–33. https://doi.org/10.1016/j.brainres.2011.12.042
McElroy B, Zakaria A, Glass JD, Prosser RA (2009) Ethanol modulates mammalian circadian clock phase resetting through extrasynaptic GABA receptor activation. Neuroscience 164(2):842–848 Retrieved from PM:19695310 http://ac.els-cdn.com/S0306452209013384/1-s2.0-S0306452209013384-main.pdf?_tid=058c842e-453e-11e6-8cf5-00000aab0f6b&acdnat=1468004516_e8a42238ce6fb3bb28474108cae42a95
Melon LC, Boehm SL 2nd. (2011) GABAA receptors in the posterior, but not anterior, ventral tegmental area mediate Ro15-4513-induced attenuation of binge-like ethanol consumption in C57BL/6J female mice. Behav Brain Res 220(1):230–237. https://doi.org/10.1016/j.bbr.2011.02.014
Mohler H (2015) The legacy of the benzodiazepine receptor: from flumazenil to enhancing cognition in Down syndrome and social interaction in autism. Adv Pharmacol 72:1–36. https://doi.org/10.1016/bs.apha.2014.10.008
Morin LP, Wood RI (2001) A stereotaxic atlas of the golden hamster brain. Academic Press, San Diego and London
Morrison KE, Bader LR, Clinard CT, Gerhard DM, Gross SE, Cooper MA (2014) Maintenance of dominance status is necessary for resistance to social defeat stress in Syrian hamsters. Behav Brain Res 270:277–286. https://doi.org/10.1016/j.bbr.2014.05.041
Mortensen M, Ebert B, Wafford K, Smart TG (2010) Distinct activities of GABA agonists at synaptic- and extrasynaptic-type GABAA receptors. J Physiol 588(Pt 8):1251–1268. https://doi.org/10.1113/jphysiol.2009.182444
Nelson RJ, Trainor BC (2007) Neural mechanisms of aggression. Nat Rev Neurosci 8(7):536–546. https://doi.org/10.1038/nrn2174
Potegal M, Perumal AS, Barkai AI, Cannova GE, Blau AD (1982) GABA binding in the brains of aggressive and non-aggressive female hamsters. Brain Res 247(2):315–324 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/6889905
Potegal M, Yoburn B, Glusman M (1983) Disinhibition of muricide and irritability by intraseptal muscimol. Pharmacol Biochem Behav 19(4):663–669 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/6685877
Primus RJ, Kellogg CK (1991) Experience influences environmental modulation of function at the benzodiazepine (BZD)/GABA receptor chloride channel complex. Brain Res 545(1-2):257–264 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/1713526
Risold PY, Swanson LW (1997a) Chemoarchitecture of the rat lateral septal nucleus. Brain Res Brain Res Rev 24(2-3):91–113 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/9385453
Risold PY, Swanson LW (1997b) Connections of the rat lateral septal complex. Brain Res Brain Res Rev 24(2-3):115–195 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/9385454
Ross AP, Norvelle A, Choi DC, Walton JC, Albers HE, Huhman KL (2017) Social housing and social isolation: impact on stress indices and energy balance in male and female Syrian hamsters (Mesocricetus auratus). Physiol Behav 177:264–269. https://doi.org/10.1016/j.physbeh.2017.05.015
Saldivar-Gonzalez A, Gomez C, Martinez-Lomeli I, Arias C (2000) Effect of flumazenil and diazepam on transient actions in defensive burying elicited by the social interaction experience in rats. Pharmacol Biochem Behav 66(2):265–273 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10880678
Serra M, Mostallino MC, Talani G, Pisu MG, Carta M, Mura ML, ..., Biggio G (2006). Social isolation-induced increase in alpha and delta subunit gene expression is associated with a greater efficacy of ethanol on steroidogenesis and GABA receptor function. J Neurochem, 98(1), 122–133. doi:https://doi.org/10.1111/j.1471-4159.2006.03850.x
Sesarini CV, Costa L, Naymark M, Granana N, Cajal AR, Garcia Coto M et al (2014) Evidence for interaction between markers in GABA(A) receptor subunit genes in an Argentinean autism spectrum disorder population. Autism Res 7(1):162–166. https://doi.org/10.1002/aur.1353
Sieghart W, Sperk G (2002) Subunit composition, distribution and function of GABA(A) receptor subtypes. Curr Top Med Chem 2(8):795–816 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12171572
Sigel E, Steinmann ME (2012) Structure, function, and modulation of GABA(A) receptors. J Biol Chem 287(48):40224–40231. https://doi.org/10.1074/jbc.R112.386664
Simler S, Puglisi-Allegra S, Mandel P (1982) Gamma-aminobutyric acid in brain areas of isolated aggressive or non-aggressive inbred strains of mice. Pharmacol Biochem Behav 16(1):57–61 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/7199186
Snyder-Mackler N, Sanz J, Kohn JN, Brinkworth JF, Morrow S, Shaver AO, ..., Tung J (2016). Social status alters immune regulation and response to infection in macaques. Science, 354(6315), 1041–1045. doi:https://doi.org/10.1126/science.aah3580
Suomi SJ, Harlow HF, Kimball SD (1971) Behavioral effects of prolonged partial social isolation in the rhesus monkey. Psychol Rep 29(3):1171–1177. https://doi.org/10.2466/pr0.1971.29.3f.1171
Swanson LW, Cowan WM (1979) The connections of the septal region in the rat. J Comp Neurol 186(4):621–655. https://doi.org/10.1002/cne.901860408
Terranova JI, Song Z, Larkin TE 2nd, Hardcastle N, Norvelle A, Riaz A, Albers HE (2016) Serotonin and arginine-vasopressin mediate sex differences in the regulation of dominance and aggression by the social brain. Proc Natl Acad Sci U S A 113(46):13233–13238. https://doi.org/10.1073/pnas.1610446113
Tremblay RE (2014) Early development of physical aggression and early risk factors for chronic physical aggression in humans. Curr Top Behav Neurosci 17:315–327. https://doi.org/10.1007/7854_2013_262
Wallner M, Hanchar HJ, Olsen RW (2014) Alcohol selectivity of beta3-containing GABAA receptors: evidence for a unique extracellular alcohol/imidazobenzodiazepine Ro15-4513 binding site at the alpha+beta- subunit interface in alphabeta3delta GABAA receptors. Neurochem Res 39(6):1118–1126. https://doi.org/10.1007/s11064-014-1243-0
Walton JC, McNeill JKT, Oliver KA, Albers HE (2017) Temporal regulation of GABAA receptor subunit expression: role in synaptic and xxtrasynaptic communication in the suprachiasmatic nucleus. eNeuro 4(2). https://doi.org/10.1523/ENEURO.0352-16.2017
Wise DA (1974) Aggression in the female golden hamster: effects of reproductive state and social isolation. Horm Behav 5(3):235–250 Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/4474137
Young LJ, Pfaff DW (2014) Sex differences in neurological and psychiatric disorders. Front Neuroendocrinol 35(3):253–254. https://doi.org/10.1016/j.yfrne.2014.05.005
Acknowledgments
We thank Dr. Joseph I. Terranova for his assistance with these experiments.
Funding
This work was supported by the National Institutes of Health under award number F31MH113367 to JMB, F32NS092545 to JCW, and R01MH109302 and R01MH110212 to HEA and funds from the Brains and Behavior Program at Georgia State University.
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Borland, J.M., Walton, J.C., Norvelle, A. et al. Social experience and sex-dependent regulation of aggression in the lateral septum by extrasynaptic δGABAA receptors. Psychopharmacology 237, 329–344 (2020). https://doi.org/10.1007/s00213-019-05368-z
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DOI: https://doi.org/10.1007/s00213-019-05368-z