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Exploring the dynamics of toxigenic environmental Vibrio mimicus and its comparative analysis with Vibrio cholerae of the southern Gangetic delta

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Abstract

Vibrio mimicus and Vibrio cholerae are closely related species. Environmental V.mimicus were comparatively analyzed with V.cholerae, for the presence of virulence genes, antibiotic susceptibility, resistance genes, in-vitro hemolysis, and biofilm formation. Phylogenetic analysis was performed depending on toxin-gene disposition and isolation area. One V.mimicus isolate harbored ctxA, tcp El-Tor, toxT and toxS, whereas several strains contained incomplete copies of virulence cassettes and associated toxin genes. V.cholerae isolates harbored ctx, tcp and toxT genes, with a higher preponderance of hlyA, rtxA and toxR genes. V.mimicus were highly sensitive to amino/carboxy-penicillins, furazolidone & gentamycin, with quinolone & tetracycline resistance genes. V.cholerae isolates were sensitive to penicillins and cephalosporins, with 29% of the strains bearing the sxt gene. Phylogenetically, the apomorphic strains of both species were unique to the inland sites. V.cholerae has embodied an enormous public health burden globally but our findings emphasize the role of V.mimicus as an emerging etiological agent with similar epidemic potential.

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Acknowledgements

Madhumanti Halder (SRF) was financially supported by fellowships from UGC, Govt. of India. Suvajit Saha (JRF) was financially supported by fellowships from MoES, Govt. of India (grant No. MoES/36/OOIS/Extra/37/2013). Subham Mookerjee (SRF) was financially supported by fellowships from CSIR, Govt. of India (File No. 09/482[0062]/2015-EMR-I).

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This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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  1. Division of Bacteriology, Indian Council of Medical Research- National Institute of Cholera & Enteric Diseases, P- 33, Scheme-XM, CIT Road, Beliaghata, Kolkata, 700 010, India

    Madhumanti Halder, Suvajit Saha, Subham Mookerjee & Anup Palit

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  1. Madhumanti Halder
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Contributions

M.H. and A.P. conceived and designed the study approach; M.H. and S.S. performed the experiments; M.H., S.M. and S.S. analyzed the data; M.H. drafted the paper and all co-authors reviewed and revised the final manuscript.

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Correspondence to Anup Palit.

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Halder, M., Saha, S., Mookerjee, S. et al. Exploring the dynamics of toxigenic environmental Vibrio mimicus and its comparative analysis with Vibrio cholerae of the southern Gangetic delta. Arch Microbiol 204, 420 (2022). https://doi.org/10.1007/s00203-022-03028-z

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