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LuxS quorum sensing system mediating Lactobacillus plantarum probiotic characteristics

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Abstract

Lactobacillus plantarum is one of common probiotics in fermented foods. Quorum sensing (QS) is a common communication way within bacteria. It is not clear whether the probiotic properties of L. plantarum mediated by QS. Here, Lb. plantarum YM-4–3 was examined for resistance of pH, bile, antimicrobial and luxS gene expression pattern. The study found that: (1) the supernatant of YM-4–3 had bacteriostatic effect to Escherichia coli O157:H7, Listeria monocytogenes and Staphylococcus aureus; (2) Lb. plantarum YM-4–3 shown tolerance property to the strongest acid culture that pH value of 3; (3) the bile tolerance of Lb. plantarum YM-4–3 was significant difference with the growth stage, the early exponential phase of the growth culture can tolerate bile of 0.4% (w/v), while the stationary growth stage can only tolerate bile of 0.2%; (4) Lb. plantarum YM-4–3 luxS gene was contrary expression along with the growth. (5) Compared with the wild-type strain, the adhesion ability of Lb. plantarum YM-4–3 ΔluxS was decreased obviously. These results showed that AI-2 LuxS quorum sensing system mediating Lb. plantarum acid, bile tolerance, antimicrobial and adhesion of probiotics.

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Acknowledgements

This work was supported by the Young Academic and Technical Leader Raising Foundation of Yunnan province (to Yiyong Luo), the Ten-thousand Talents Program in Yunnan province (to Yiyong Luo). Sponsored by K.C. Wong Magna Fund at Ningbo University and General project of Zhejiang Department of Education (Y202043742). This work was supported by National Natural Science Foundation of China (Grant nos. 31300068 and 31660451).

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Author notes

  1. Liming Jiang, Xuebin Cao and Wen Liu have contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo, 315211, People’s Republic of China

    Liming Jiang & Xuebin Cao

  2. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, People’s Republic of China

    Liming Jiang & Yiyong Luo

  3. Yantai Jinghai Marine Fisheries Co., Ltd, Yantai, People’s Republic of China

    Xuebin Cao

  4. Department of Rheumatology Immunology, The First People’s Hospital of Hefei, 230061, Hefei, People’s Republic of China

    Wen Liu

  5. Longhaixingang Science and Technology Co, Ltd, 121000, Jinzhou, People’s Republic of China

    Gang Song

  6. Lanzhou Inspection and Quarantine Co, Ltd, 730070, Lanzhou, People’s Republic of China

    Zhizhen Zhang

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  1. Liming Jiang
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  2. Yiyong Luo
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  4. Wen Liu
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Correspondence to Yiyong Luo.

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Ethics statement

Ethical approval was obtained from the Institutional Ethics Committee (Kunming University of science and technology, Kunming, Yunnan, China). The study protocol was in accordance with the Declaration of Helsinki for Human Research of 1974 (last modified in 2000).

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Communicated by Erko Stackebrandt.

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Jiang, L., Luo, Y., Cao, X. et al. LuxS quorum sensing system mediating Lactobacillus plantarum probiotic characteristics. Arch Microbiol 203, 4141–4148 (2021). https://doi.org/10.1007/s00203-021-02404-5

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