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Nonomuraea phyllanthi sp. nov., an endophytic actinomycete isolated from the leaf of Phyllanthus amarus

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Abstract

A novel actinomycete, strain PA1-10T, isolated from the leaf of Phyllanthus amarus collected from Bangkok, Thailand, was characterized taxonomically using a polyphasic approach. This strain contained the characteristics consistent with those of members of the genus Nonomuraea. It formed short rugose spore chain on aerial mycelium. The diamino acid in cell wall peptidoglycan was meso-diaminopimelic acid. Galactose, glucose, madurose, mannose, and ribose were found in whole-cell hydrolysates. Predominant menaquinones were MK-9 (H2), MK-9 (H4), and MK-9 (H6). Major cellular fatty acids were iso-C16:0 and C17:0 10-methyl. Phospholipid profiles were composed of phosphatidylinositol mannoside (PIM), lyso-phosphatidylethanolamine (lyso-PE), phosphatidylethanolamine (PE), methylphosphatidylethanolamine (PME), diphosphatidylglycerol (DPG), and phosphatidylglycerol (PG). The G + C content of DNA was 71.2 mol%. Strain PA1-10T showed the highest 16S rRNA gene sequence similarity with Nonomuraea candida JCM 15928T (98.35%) and shared the same node with Nonomuraea maritima JCM 18321T in the phylogenetic tree analysis. Based on the phenotypic characteristics, DNA–DNA relatedness, and average nucleotide identity (ANI), the strain is considered to represent a novel species of the genus Nonomuraea, for which the name Nonomuraea phyllanthi is proposed. The type strain is PA1-10T (= JCM 33073T = NBRC 112774T = TISTR 2497T).

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Acknowledgements

We would like to thank the Associate Professor Thatree Phadungcharoen, the Faculty of Pharmaceutical Sciences, Chulalongkorn University for the plant sample and the Pharmaceutical Research Instrument Center, Faculty of Pharmaceutical Sciences, Chulalongkorn University, for providing research facilities.

Funding

This study was supported by the Thailand Research Fund for a 2017 Royal Golden Jubilee Ph.D. Program as a scholarship to N. K. and the Grant for International Research Integration, Research Pyramid, Ratchadaphiseksomphot Endowment Fund (GCURP_58_01_33_01), Chulalongkorn University.

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Authors and Affiliations

  1. Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand

    Nattaporn Klykleung & Somboon Tanasupawat

  2. Japan Collection of Microorganisms, RIKEN BioResource Center, Ibaraki, 305-0074, Japan

    Masahiro Yuki, Takuji Kudo & Moriya Ohkuma

  3. Department of Biology, Faculty of Science, Ramkhamhaeng University, Bangkok, 10240, Thailand

    Wongsakorn Phongsopitanun

  4. National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathum Thani, 12120, Thailand

    Pattama Pittayakhajonwut

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  1. Nattaporn Klykleung
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  2. Masahiro Yuki
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Correspondence to Somboon Tanasupawat.

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Communicated by Erko Stackebrandt.

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Klykleung, N., Yuki, M., Kudo, T. et al. Nonomuraea phyllanthi sp. nov., an endophytic actinomycete isolated from the leaf of Phyllanthus amarus. Arch Microbiol 202, 55–61 (2020). https://doi.org/10.1007/s00203-019-01717-w

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