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Investigation of the effect of different environmental conditions on biofilm structure of Salmonella enterica serotype Virchow via FTIR spectroscopy

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Abstract

This study aims to describe the content of polymeric matrix components under different incubation temperatures and pH levels. Optimal biofilm production of 15 S. Virchow isolates occurred following the incubation in LB−NaCl for 72 h, at pH 6.6 and 20 °C. The expression of csgA, csgD, adrA and bcsA genes at 20 °C, 25 °C and 30 °C in S. Virchow DMC18 was analyzed, and it was discovered that the maximum production of cellulose and curli fimbriae occurred at 20 °C. The physical characteristics of pellicle structure of S. Virchow DMC18 was determined as rigid at 20 °C, while becoming fragile at higher temperatures. FTIR analyses confirmed the obtained molecular findings. The intensities of the 16 different peaks originating from carbohydrate, protein, and nucleic acid in the spectra of biofilm samples significantly diminished (p < 0.05) with the increasing temperature. The highest intensities of lipids and carbohydrates were observed at 20 °C indicating the changes in cell surface properties.

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Authors and Affiliations

  1. Biotechnology Institute, Ankara University, Ankara, Turkey

    Mohammad Nima Ariafar & Nefise Akçelik

  2. Department of Molecular Biology and Genetics, Nevşehir Hacı Bektaş Veli University, Nevşehir, Turkey

    Nasit Iğci

  3. Biology Department, Faculty of Science, Ankara University, Ankara, Turkey

    Mustafa Akçelik

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  1. Mohammad Nima Ariafar
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  2. Nasit Iğci
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  4. Nefise Akçelik
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Correspondence to Nefise Akçelik.

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Author contributions

NA: coordinated and drafted the manuscript, carried out the experiments. Nİ: performed the FTIR experiments and participated in the interpretation of the FTIR results. NA* and MA conceived of the study and planned the experiments, supervised the project and took the lead in the writing the manuscript. All the authors provided critical feedback and helped the research, analysis and manuscript.

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Communicated by Erko Stackebrandt.

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Ariafar, M.N., Iğci, N., Akçelik, M. et al. Investigation of the effect of different environmental conditions on biofilm structure of Salmonella enterica serotype Virchow via FTIR spectroscopy. Arch Microbiol 201, 1233–1248 (2019). https://doi.org/10.1007/s00203-019-01681-5

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