Skip to main content
Log in

Whole-genome sequence and genome annotation of Xanthomonas citri pv. mangiferaeindicae, causal agent of bacterial black spot on Mangifera indica

  • Short Communication
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

A newly isolated strain XC01 was identified as Xanthomonas citri pv. mangiferaeindicae, isolated from an infected mango fruit in Guangxi, China. The complete genome sequence of XC01 was carried out using the PacBio RSII platform. The genome contains a circular chromosome with 3,865,165 bp, 3442 protein-coding genes, 53 tRNAs, and 2 rRNA operons. Phylogenetic analysis revealed that this pathogen is very close to the soybeans bacterial pustule pathogen X. citri pv. glycines CFBP 2526, with a completely different host range. The genome sequence of XC01 may shed a highlight genes with a demonstrated or proposed role in on the pathogenesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

References

  • Apweiler R, Bairoch A, Wu C, Yeh LL (2004) UniProt: the universal protein knowledgebase. Nucleic Acids Res 32:115–119

    Article  CAS  Google Scholar 

  • Ashburner M, Ball CA, Blake JA, Botstein D et al (2000) Gene ontology: tool for the unification of biology. Nat Genetics 25(1):25–29

    Article  PubMed  CAS  Google Scholar 

  • Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA et al (2008) The RAST server: rapid annotations using subsystems technology. BMC Genom 9:75

    Article  CAS  Google Scholar 

  • Chin CS, Alexander DH, Marks P, Klammer AA, Drake J, Heiner C et al (2013) Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data. Nat Methods 10:563–569

    Article  PubMed  CAS  Google Scholar 

  • Clarke CR, Cai R, Studholme DJ, Guttman DS, Vinatzer BA (2010) Pseudomonas syringae strains naturally lacking the classical P. syringae hrp/hrc locus are common leaf colonizers equipped with an atypical type III secretion system. Mol Plant Microbe Interact 23:198–210

    Article  PubMed  CAS  Google Scholar 

  • Cummings MP (2014) PAUP*(Phylogenetic analysis using parsimony (*and other methods)). 4.0 ed. Sinauer Associates, Sunderland

    Google Scholar 

  • Darrasse A, Bolot S, Serres-Giardi L, Charbit E, Boureau T, Fisher-Le Saux M, Briand M, Arlat M, Gagnevin L, Koebnik R, Noël LD, Carrère S, Jacques MA (2013) High-quality draft genome sequences of Xanthomonas axonopodis pv. glycines strains CFBP 2526 and CFBP 7119. Genome Announcements 1(6):e01036–13

    Article  PubMed  PubMed Central  Google Scholar 

  • Gagnevin L, Pruvost O (2001) Epidemiology and control of mango bacterial black spot. Plant Dis 85(9):928–935

    Article  Google Scholar 

  • Hyatt D, Chen GL, Locascio PF, Land ML, Larimer FW, Hauser LJ, (2010) Prodigal: prokaryotic gene recognition and translation initiation site identification. BMC Bioinform. 11:119

    Article  CAS  Google Scholar 

  • Kanehisa M, Goto S, Kawashima S, Hattori M (2004) The KEGG resource for deciphering the genome. Nucleic Acids Res 32:277–280

    Article  CAS  Google Scholar 

  • Lagesen K, Hallin PF, Rødland E, Stærfeldt HH, Rognes T, Ussery DW (2007) RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Nucleic Acids Res 35:3100–3108

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F et al. (2007) ClustalW2 and ClustalX version 2. Bioinformatics 23:2947–2948

    Article  PubMed  CAS  Google Scholar 

  • Li L, Stoeckert CJ, Roos DS (2003) OrthoMCL: identification of ortholog groups for eukaryotic genomes. Genom Res 13:2178–2189

    Article  CAS  Google Scholar 

  • Lowe TM, Eddy SR (1997) tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res 25:955–964

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Ochiai H, Inoue Y, Takeya M, Sasaki A, Kaku H (2005) Genome sequence of Xanthomonas oryzae pv. oryzae suggests contribution of large numbers of effector genes and insertion sequences to its race diversity. JARQ 39(4):275–287

    Article  CAS  Google Scholar 

  • Palmer LE, McCombie. WR (2002) On the importance of being finished. Genom Biol 3:201

    Article  Google Scholar 

  • Patel MK, Moniz L, Kulkarni YS (1948) A new bacterial disease of Mangifera indica L. Curr Sci 17(6):189–190

    PubMed  CAS  Google Scholar 

  • Ploetz R, Ploetz R (2003) Diseases of tropical fruit crops. CABI Publishing, pp 327–363

  • Ploetz RC, Prakash O (1997) Foliar, floral and soil borne diseases. In: Litz RE (ed) The mango: botany, production and uses. CAB International, Oxon, pp 281–327

    Google Scholar 

  • Ray SK, Rajeshwari R, Sonti RV (2000) Mutants of Xanthomonas oryzae deficient in general secretory pathway are virulent deficient and unable to secrete xylase. Mol. Plant Microbe Interact 13:394–401

    Article  CAS  Google Scholar 

  • Singh Z, Singh RK, Sane VA, Nath P (2013) Mango-postharvest biology and biotechnology. Crit Rev Plant Sci 32(4):217–236

    Article  CAS  Google Scholar 

  • Tatusov RL, Galperin MY, Natale DA, Koonin EV (2000) The COG database: a tool for genome-scale analysis of protein functions and evolution. Nucleic Acids Res 28(1):33–36

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Triplett LR, Hamilton JP, Buell CR, Tisserat NA, Verdier V, Zink F, Leach JE (2011) Genomic analysis of Xanthomonas oryzae isolates from rice grown in the United States reveals substantial divergence from known X. oryzae pathovars. Appl Environ Microbiol 77:3930–3937

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Vauterin L, Hoste B, Kersters K, Swings J (1995) Reclassification of xanthomonas. Int J Syst Bacteriol 45:472–489

    Article  CAS  Google Scholar 

  • Winnenburg R, Baldwin TK, Urban M, Rawlings C, Kohler J, Hammond-Kosack KE (2006). PHI-base: a new database for pathogen host interactions. Nucleic Acids Res. 34:459–464

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research was supported by the Chinese Special Fund of Basic Scientific Research Projects for State Level and Public Welfare-Scientific Research Institutes (No. 1630062014007), the special fund for Agroscientific Research in the Public Interest (No. 201203092), and Hainan Province Science Foundation (No. 20163109).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Feng Liu.

Additional information

Communicated by Eriko Takano.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Liu, F., Ou, Xc. & Zhan, Rl. Whole-genome sequence and genome annotation of Xanthomonas citri pv. mangiferaeindicae, causal agent of bacterial black spot on Mangifera indica. Arch Microbiol 200, 835–840 (2018). https://doi.org/10.1007/s00203-018-1513-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00203-018-1513-3

Keywords

Navigation