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Boosting the growth of the probiotic strain Lactobacillus paracasei ssp. paracasei F19

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Abstract

Single so-called booster substances were added to the fermentation medium of the probiotic strain Lactobacillus (L.) paracasei ssp. paracasei F19 to enhance its growth. A wide screening was carried out in microtiter plates and a statistical analysis of the growth parameters was performed. CFU counts were used to correlate the increase in OD590nm with the increase in viable cell number. Sodium ascorbate, sodium pyruvate, manganese sulfate and cysteine had a remarkable boosting effect on the growth of L. paracasei F19. Three of the boosters increased the growth rate of the strain and led to a higher cell density and biomass yield in laboratory conditions. Cysteine significantly shortened the lag phase, therefore reducing the fermentation times. The boosters were tested on four additional Lactobacillus species and their growth boosting activity was retained. To investigate whether the growth boosters could improve the tolerance of L. paracasei F19 to the adverse condition in the GI tract, additional tests were performed. Sodium ascorbate and sodium pyruvate exerted a certain antioxidant effect, as they improved the tolerance of L. paracasei F19 to H2O2. Sodium ascorbate enhanced the growth of the strain in low pH.

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Author notes

  1. Jürgen Behr

    Present address: Bavarian Center for Biomolecular Mass Spectrometry, Technische Universität München, Gregor-Mendel Str. 4, 85354, Freising, Germany

Authors and Affiliations

  1. Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Freising, Germany

    Desideria Brignone, Pia Radmann, Jürgen Behr & Rudi F. Vogel

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  1. Desideria Brignone
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  2. Pia Radmann
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  3. Jürgen Behr
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  4. Rudi F. Vogel
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Correspondence to Jürgen Behr.

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Funding

Parts of the presented work were funded by the German Federal Office of Agriculture and Food in project BLE 2817400111, http://www.ble.de. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Communicated by Erko Stackebrandt.

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Brignone, D., Radmann, P., Behr, J. et al. Boosting the growth of the probiotic strain Lactobacillus paracasei ssp. paracasei F19. Arch Microbiol 199, 853–862 (2017). https://doi.org/10.1007/s00203-017-1352-7

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  • DOI: https://doi.org/10.1007/s00203-017-1352-7

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