Abstract
Se (Selenium) has been reported to be an important protective agent to decreases Cd (Cadmium) induced toxic in plants. However, it remains unclear how Se mitigates the uptake of Cd and increased the resistance to Cd toxicity. Hydroponic experiments were arranged to investigate the changes of physiological properties, root cell membrane integrity and Cd-related transporter genes in rape seedlings. Comparison of the biomass between the addition of Se and the absence of Se under Cd exposure showed that the Cd-induced growth inhibition of rape seedlings was alleviated by Se. Cd decreased the photosynthetic rate (Pn), stomatal conductance (Gs) and photosynthetic pigment content including chlorophyll a, chlorophyll b and carotenoid. However, all these parameters were all significantly improved by Se addition. Moreover, exposure to Se resulted in a decrease in Cd concentration in both shoot and root, ranging from 4.28 to 27.2%. Notably, the application of Se at a concentration of 1 µmol L− 1 exhibited the best performance. Furthermore, Se enhanced cell membrane integrity and reduced superoxide anion levels, thereby contributing to the alleviation of cadmium toxicity in plants. More critically, Se decreased the expression levels of root Cd-related transporter genes BnIRT1, BnHMA2 and BnHMA4 under Cd stress, which are responsible for Cd transport and translocation. These results are important to increase crop growth and reduce Cd load in the food chain from metal toxicity management and agronomical point of view.
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Acknowledgements
This work is funded by Fujian Provincial Key Lab of Coastal Basin Environment (Fujian Polytechnic Normal University) (S1-KF2110), the Opening Project of Fujian Provincial Key Laboratory of Resources and Environment Monitoring & Sustainable Management and Utilization (ZD202101), Program for Innovative Research Team in Science and Technology in Fujian Province University (KC190002), the Opening Project of Key Laboratory of Risk Assessment for Agro-product Quality and Safety, Guangdong Province (SZKF202101).
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Yuanyuan Zhao and Yanni Tang conceived the experiment, performed the main experiments and data analysis, and wrote the manuscript. Yingjie Zhou, Shiqian Li, Chihhung Wu, Guangyu Shi and Chengxiao Hu assisted in the implementation of the experiment and data analysis. Xiaohu Zhao conceived the experiment and revised the manuscript. All authors have approved the final version of the manuscript.
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Tang, Y., Zhao, Y., Zhou, Y. et al. Se Ameliorates Cd Toxicity in Oilseed rape (Brassica napus L.) Seedlings by Inhibiting Cd Transporter Genes and Maintaining root Plasma Membrane Integrity. Bull Environ Contam Toxicol 111, 42 (2023). https://doi.org/10.1007/s00128-023-03804-7
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DOI: https://doi.org/10.1007/s00128-023-03804-7