Abstract
Polychlorinated biphenyls (PCBs) are released into the environment from a wide range of sources. The aim of the present study was to investigate the effect of the PCBs extracted from the Zhanjiang mangrove sediments on the immune function of zebrafish. The sediments were collected from 3 mangrove forest points in Zhanjiang (Guangdong Province, China), and the results showed that PCB153 was detected in the sediments of the Guangdong Zhanjiang Mangrove National Nature Reserve (MNNR) and Gaoqiao Mangrove Reserve (GMR), while PCB101, PCB112, PCB155, and PCB198 were detected in the sediments of the Leizhou Peninsula (LP). The zebrafish were exposed to different concentrations of PCBs, i.e., control group, positive control group (Aroclor1254; 10 μg/L), low dose group (LD; 0.6 μg/L), medium-dose group (MD; 3.0 μg/L) and high dose group (HD; 15 μg/L) for 14 days. As compared to the control group, the liver index increased significantly in all PCB treated groups. The liver tissue structure was destroyed in all PCB-treated groups as compared to the control group. In addition, the relative mRNA expression of the target genes (IL-1β, IL-8, and TNF-α) was significantly expressed in each concentration group. Therefore, these findings suggest that exposure of zebrafish to PCBs can destroy the liver histology and increase the liver index and mRNA expression of inflammatory cytokines in a dose and time-dependent manner.
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Acknowledgements
I acknowledge the cooperative spirit of all my colleagues who helped me on this project.
Funding
This study was financially supported by the Marine Economy Development Project of Guangdong Province for High-Quality Economic Development (GDOE2019A52).
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JC, GHA, and RG conceived the idea and planned the project. DK, FN, and ZC participated in its design and coordination. SGM collected the data. AQ performed the experiment and wrote the first draft of the manuscript. JC and JW edited the manuscript. All authors read and approved the final manuscript.
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According to rules and regulations of the Animal Care Committee of Guangdong Ocean University (Zhanjiang, Guangdong, People’s Republic of China).
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Qamar, A., Waheed, J., Ghulam Mohyuddin, S. et al. The Status of Polychlorinated Biphenyls (PCBs) Extract from Zhanjiang Mangrove Sediments and the Effects on Tissue Structure and Inflammatory Cytokines in Zebrafish Liver. Bull Environ Contam Toxicol 108, 890–900 (2022). https://doi.org/10.1007/s00128-021-03439-6
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DOI: https://doi.org/10.1007/s00128-021-03439-6