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Accumulation and Depuration of Cd and its Effect on the Expressions of Metallothionein and Apoptotic Genes in Litopenaeus vannamei

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Abstract

We investigated cadmium (Cd) accumulation in muscles, gills and hepatopancreas of Litopenaeus vannamei following 48 h exposure to 5.25 mg/L, and depuration of Cd in these tissues on 1, 5 and 15 d post exposure. We also detected the expressions of metallothionein (MT), caspase-3 and p53 in hepatopancreas of shrimp exposed to 0, 5.25 and 10.5 mg/L Cd (the 24 h median lethal concentration, 24 h LC50) at 0, 3, 12, 24 and 48 h. Cd accumulated with high concentration in hepatopancreas, and low concentration in muscles. Cd depurated fast in hepatopancreas and gills. MT expression increased in a time-dependent manner after Cd exposure. The p53 and caspase-3 increased at 12 and 24 h in 10.5 mg/L group. In conclusion, the accumulation and depuration of Cd in three tissues were tissues-specific. The changes of the expressions of MT, p53 and caspase-3, were stress response of L. vannamei under Cd exposure.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (31600321).The authors would like to thank teacher Qitao Zeng and student Xiaoying Ru for technical assistance during experiment.

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  1. Ling Li and Yu-Chun Shen have contributed equally to this work.

Authors and Affiliations

  1. College of Fisheries, Guangdong Ocean University, Mazhang District, Haida Road No. 1, Zhanjiang, 524000, Guangdong, People’s Republic of China

    Ling Li, Yu-Chun Shen, Jin-Rong Liang, Hu Liu, Tian-Ci Chen & Hui Guo

  2. Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China

    Hui Guo

Authors
  1. Ling Li
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  2. Yu-Chun Shen
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  3. Jin-Rong Liang
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  4. Hu Liu
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  5. Tian-Ci Chen
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  6. Hui Guo
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Correspondence to Hui Guo.

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Li, L., Shen, YC., Liang, JR. et al. Accumulation and Depuration of Cd and its Effect on the Expressions of Metallothionein and Apoptotic Genes in Litopenaeus vannamei. Bull Environ Contam Toxicol 106, 501–506 (2021). https://doi.org/10.1007/s00128-021-03115-9

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  • DOI: https://doi.org/10.1007/s00128-021-03115-9

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