Skip to main content
SpringerLink
Log in

Identification of two loci for resistance to clubroot (Plasmodiophora brassicae Woronin) in Brassica rapa L.

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

In an analysis of 114 F2 individuals from a cross between clubroot-resistant and susceptible lines of Brassica rapa L., 'G004' and 'Hakusai Chukanbohon Nou 7' (A9709), respectively, we identified two loci, Crr1 and Crr2, for clubroot (caused by Plasmodiophora brassicae Woronin) resistance. Each locus segregated independently among the F2 population, indicating that the loci reside on a different region of chromosomes or on different chromosomes. Genetic analysis showed that each locus had little effect on clubroot resistance by itself, indicating that these two loci are complementary for clubroot resistance. The resistance to clubroot was much stronger when both loci were homozygous for resistant alleles than when they were heterozygous. These results indicate that clubroot resistance in B. rapa is under oligogenic control and at least two loci are necessary for resistance.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.

Similar content being viewed by others

References

  • Bell CJ, Ecker JR (1994) Assignment of 30 microsatellite loci to the linkage map of Arabidopsis. Genomics 19:137–144

    Article  CAS  PubMed  Google Scholar 

  • Bittner-Eddy PD, Crute IR, Holub EB, Beynon JL (2000) RPP13 is a simple locus in Arabidopsis thaliana for alleles that specify downy mildew resistance to different avirulence determinants in Peronospora parasitica. Plant J 21:177–188

    Article  CAS  PubMed  Google Scholar 

  • Botella MA, Coleman MJ, Hughes DE, Nishimura MT, Jones JDG, Somerville SC (1997) Map positions of 47 Arabidopsis sequences with sequence similarity to disease-resistant genes. Plant J 12:1197–1211

    PubMed  Google Scholar 

  • Buczacki ST, Toxopeus H, Mattusch P, Johnston TD, Dixon GR, Hobolth LA (1975) Study of physiologic specialization in Plasmodiophora brassicae: proposals for rationalization through an international approach. Trans Br Mycol Soc 65:295–303

    Google Scholar 

  • Chiang BY, Chiang MS, Grant WF, Crete R (1980) Transfer of resistance to race 2 of Plasmodiophora brassicae from Brassica napus to cabbage (B. oleracea spp. capitata). IV. A resistant 18-chromosome B1 plant and its B2 progenies. Euphytica 29:47–55

    Google Scholar 

  • Crute IR, Pink DAC (1996) Genetic and utilization of pathogen resistance in plants. Plant Cell 8:1747–1755

    CAS  Google Scholar 

  • Crute IR, Gray AR, Crisp P, Buczacki ST (1980) Variation in Plasmodiophora brassicae and resistance to clubroot disease in brassicas and allied crops – a critical review. Plant Breed Abstr 50:91–104

    Google Scholar 

  • Dib C, Faure S, Fizames C, Samson D, Drouot N, Vignal A (1996) A comprehensive genetic map of the human genome based on 5,264 microsatellites. Nature 380:152–154

    PubMed  Google Scholar 

  • Dietrich WF, Miller J, Steen R, Merchant MA, Damron-Boles D, Husain Z (1996) A comprehensive genetic map of the mouse genome. Nature 380:149–152

    CAS  PubMed  Google Scholar 

  • Figdore SS, Ferreira ME, Slocum MK, Williams PH (1993) Association of RFLP markers with trait loci affecting clubroot resistance and morphological characters in Brassica oleracea L. Euphytica 69:33–44

    CAS  Google Scholar 

  • Ford CM, Paltridge NG, Rathjen JP, Moritz RL, Simpson RJ, Symons RH (1998) Rapid and informative assays for Yd2, the barley yellow dwarf virus resistance gene, based on the nucleotide sequence of a closely linked gene. Mol Breed 4:23–31

    Article  CAS  Google Scholar 

  • Fuchs H, Sacristán MD (1996) Identification of a gene in Arabidopsis thaliana controlling resistance to clubroot (Plasmodiophora brassicae) and characterization of the resistance response. Mol Plant-Microbe Interact 9:91–97

    Google Scholar 

  • Grandclément C, Thomas G (1996) Detection and analysis of QTLs based on RAPD markers for polygenic resistance to Plasmodiophora brassicae Woron in Brassica oleracea L. Theor Appl Genet 93:86–90

    Article  Google Scholar 

  • Gupta PK, Varshney RK (2000) The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat. Euphytica 113:163–185

    Article  CAS  Google Scholar 

  • Hancock JM (1995) The contribution of slippage-like processes to genome evolution. J Mol Evol 41:1038–1047

    CAS  PubMed  Google Scholar 

  • Hayashi T, Ukai Y (1999) Method of QTL mapping in an F2 population using phenotypic means of F3 lines. Breed Sci 49:105–114

    CAS  Google Scholar 

  • Jones DR, Ingram DS, Dixon GR (1982) Characterization of isolates derived from single resting spores of Plasmodiophora brassicae and studies of their interaction. Plant Pathol 31:239–246

    Google Scholar 

  • Kandemir N, Jones BL, Wesenberg DM, Ullrich SE, Kleinhofs A (2000) Marker-assisted analysis of three grain yield QTLs in barley (Hordeum vulgare L.) using near-isogenic lines. Mol Breed 6:157–167

    Article  CAS  Google Scholar 

  • Karling JS (1968) The Plasmodiophorales. Hafner Publishing Company, New York

  • Kikuchi M, Ajisaka H, Kuginuki Y, Hirai M (1999) Conversion of RAPD markers for a clubroot resistance gene of Brassica rapa into sequence-tagged sites (STSs). Breed Sci 49:83–88

    CAS  Google Scholar 

  • Kresovich S, Szewc-McFadden AK, Bliek SM, McFerson JR (1995) Abundance and characterization of simple-sequence repeats (SSRs) isolated from a size-fractionated genomic library of Brasica napus L. (rapessed). Theor Appl Genet 91:206–211

    CAS  Google Scholar 

  • Kuginuki Y, Ajisaka H, Yui M, Yoshikawa H, Hida K, Hirai M (1997) RAPD markers linked to a clubroot-resistance locus in Brassca rapa L. Euphytica 98:149–154

    Article  CAS  Google Scholar 

  • Kuginuki Y, Yoshikawa H, Hirai M (1999) Variation in virulence of Plasmodiophora brassicae in Japan tested with clubroot-resistant cultivars of Chinese cabbage (Brassica rapa L. spp. pekinensis). Eur J Plant Pathol 105:327–332

    Article  Google Scholar 

  • Landry BS, Hubert N, Crête R, Chang MS, Lincoln SE, Etoh T (1992) A genetic map of Brassica oleracea based on RFLP markers detected with expressed DNA sequences and mapping of resistance genes to race 2 of Plasmodiophora brassicae (Woronin). Genome 35:409–419

    CAS  Google Scholar 

  • Morgante M, Olivieri AM (1993) PCR-amplified microsatellites as markers in plant genetics. Plant J 3:175–182

    CAS  PubMed  Google Scholar 

  • Parker GD, Langridge P (2000) Development of STS marker linked to a major locus controlling flower color in wheat (Triticum aestivum L.). Mol Breed 6:169–174

    CAS  Google Scholar 

  • Speulman E, Bouchez D, Holub EB, Beynon JL (1998) Disease resistance gene homologs correlate with disease resistance loci of Arabidopsis thaliana. Plant J 14:467–474

    CAS  PubMed  Google Scholar 

  • Suwabe K, Iketani H, Nunome T, Kage T, Hirai M (2002) Isolation and characterization of microsatellites in Brassica rapa L. Theor Appl Genet 104:1092–1098

    Article  Google Scholar 

  • Tautz D, Renz M (1984) Simple sequences are ubiquitous repetitive components of eukaryotic genomes. Nucleic Acids Res 25:4127–4138

    Google Scholar 

  • Uzunova MI, Ecke W (1999) Abundance, polymorphism and genetic mapping of microsatellites in oilseed rape (Brassica napus L.). Plant Breed 118:323–326

    Article  CAS  Google Scholar 

  • Voorrips RE (1995) Plasmodiophora brassicae: aspects of pathogenesis and resistance in Brassica oleracea. Euphytica 83:139–146

    Google Scholar 

  • Voorrips RE, Jongerius MC, Kanne HJ (1997) Mapping of two genes for resistance to clubroot (Plasmodiophora brassicae) in a population of doubled-haploid lines of Brassica oleracea by means of RFLP and AFLP markers. Theor Appl Genet 94:75–82

    Article  CAS  Google Scholar 

  • Westman AL, Kresovich S (1999) Simple sequence repeat (SSR)-based marker variation in Brassica nigra genebank accessions and weed populations. Euphytica 109:85–92

    Article  CAS  Google Scholar 

  • Williams PH (1966) A system for the determination of races of Plasmodiophora brassicae that infect cabbage and rutabaga. Phytopathology 56:624–626

    Google Scholar 

  • Yoshikawa H (1981) Breeding for clubroot resistance in Chinese cabbage. In: Talekar NS, Griggs TD (eds) Chinese cabbage. Proc 1st Int Symp, Tsukuba, Japan, pp 405–413

Download references

Acknowledgements

We thank Prof. Dr. Y. Kowyama, Dr. K. Kakeda and Dr. T. Tsuchiya of Mie University for their valuable advice. We are grateful to Ms. K. Tanaka and Ms. H. Maeda for their technical assistance. This work was supported by the Cooperative System for Supporting Priority Research of the Japan Science and Technology Corporation and by grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Rice Genome Project DM-2105).

Author information

Author notes

  1. M. Hirai

    Present address: Faculty of Agriculture, University of Kyoto prefecture, Kitainaazuma, Seika, Kyoto 619-0244, Japan,

Authors and Affiliations

  1. National Institute of Vegetable and Tea Science (NIVTS), 360 Kusawa, Ano, Age, Mie 514-2392, Japan

    K. Suwabe, H. Tsukazaki, K. Hatakeyama, M. Fujimura, T. Nunome, H. Fukuoka, S. Matsumoto & M. Hirai

  2. National Agricultural Research Organization, 3-1-1 Kannon-dai, Tsukuba, Ibaraki 305-8517, Japan

    H. Iketani

Authors
  1. K. Suwabe
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Tsukazaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Iketani
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Hatakeyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Fujimura
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. T. Nunome
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. H. Fukuoka
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. S. Matsumoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. M. Hirai
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. Matsumoto.

Additional information

Communicated by H.C. Becker

Rights and permissions

Reprints and permissions

About this article

Cite this article

Suwabe, K., Tsukazaki, H., Iketani, H. et al. Identification of two loci for resistance to clubroot (Plasmodiophora brassicae Woronin) in Brassica rapa L.. Theor Appl Genet 107, 997–1002 (2003). https://doi.org/10.1007/s00122-003-1309-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-003-1309-x

Keywords

Search

Navigation