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Expression of lubricin in rat posterior mandibular condylar cartilage following functional mandibular forward repositioning

Lubricinexpression im posterioren kondylären Knorpel bei Ratten nach funktionskieferorthopädischer Unterkiefervorverlagerung

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Abstract

Purpose

The aim of the present study was to investigate the effects of mandibular forward repositioning on expression of lubricin in rat posterior condylar cartilage.

Methods

In total, fifty 5‑week-old female Sprague Dawley rats were divided randomly into experimental groups and control groups. The animals in the experimental groups were fitted with modified acrylic inclined planes to advance the mandible, whereas rats in the normal control groups were left intact. Rats were sacrificed on days 3, 7, 14, 21, and 30, and temporomandibular joint (TMJ) samples were collected. The expression of lubricin of the posterior mandibular condylar cartilage was evaluated by immunohistochemistry.

Results

In the control groups, higher expression of lubricin was observed in the proliferative zone of the posterior mandibular condylar cartilage compared with the hypertrophic zone during the experimental period. Compared with the control group, the positive signals for lubricin of the posterior mandibular condylar cartilage in the experimental animals were significantly higher on days 7, 14, and 21; however, no statistical difference was found on day 3 or 30.

Conclusions

Data analyses suggest that the bite jumping appliance temporarily enhanced lubricin expression, providing a good mechanical environment for the physiologic growth of the condyle and mandible, and contributes to TMJ remodeling by the regulation of condylar chondrocyte proliferation.

Zusammenfassung

Ziel

Ziel der vorliegenden Studie war es, die Auswirkungen der Unterkiefervorverlagerung auf die Lubricinexpression im posterioren kondylären Knorpels bei Ratten zu untersuchen.

Methoden

Insgesamt wurden fünfzig 5 Wochen alte weibliche Sprague-Dawley-Ratten nach dem Zufallsprinzip in Versuchsgruppen und Kontrollgruppen eingeteilt. Bei den Tieren in den Versuchsgruppen wurden modifizierte Acrylplatten mit schiefer Ebene eingesetzt, um eine Unterkiefervorverlagerung zu erreichen, während die Ratten in den Kontrollgruppen keine Apparaturen erhielten. Die Ratten wurden nach 3, 7, 14, 21 und 30 Tagen geopfert und Proben des Kiefergelenks (TMJ) entnommen. Die Lubricinexpression des posterioren kondylären Knorpels wurde immunohistochemisch bestimmt.

Ergebnisse

In den Kontrollgruppen wurde im experimentellen Zeitraum in der proliferativen Zone des posterioren Kondylarknorpels eine höhere Expression von Lubricin festgestellt als in der hypertrophen Zone. Im Vergleich mit der Kontrollgruppe war die Lubricinexpression im posterioren Kondylarknorpel bei den Versuchstieren nach 7, 14 und 21 Tagen signifikant höher, nach 3 bzw. 30 Tagen wurde jedoch kein statistisch signifikanter Unterschied gefunden.

Schlussfolgerungen

Die Analyse der vorliegenden Daten deutet darauf hin, dass die funktionskieferorthopädische Apparatur temporär die Lubricinexpression erhöhte und damit gute Bedingungen für das physiologische Wachstum des Unterkiefers und des Kondylus gegeben sind, da Lubricinexpression zur Remodellierung des Kiefergelenks beiträgt, indem es die Proliferation von Chondrozyten reguliert.

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Authors and Affiliations

  1. Department of Stomatology, The Third Affiliated Hospital, Sun Yat-sen University, No. 600 Tianhe Road, Guangzhou, China

    Zheng Chen, Zhihui Mai, Hongfei Lu, Lin Chen & Hong Ai

  2. Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology and Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, China

    Shaoqin Tu

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  1. Zheng Chen
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Correspondence to Hong Ai.

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C. Zheng, M. Zhihui, T. Shaoqin, L. Hongfei, C. Lin and A. Hong declare that they have no competing interests.

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Chen, Z., Mai, Z., Tu, S. et al. Expression of lubricin in rat posterior mandibular condylar cartilage following functional mandibular forward repositioning. J Orofac Orthop 80, 128–135 (2019). https://doi.org/10.1007/s00056-019-00173-x

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