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Anti-inflammatory effects of kaempferol-3-O-sophoroside in human endothelial cells

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Abstract

Background

Kaempferol-3-O-sophoroside (KPOS) was isolated from the leaves of cultivated mountain ginseng. Kaempferol (KP) has antitumor, anti-oxidative, anti-allergic and antidiabetic activities but the barrier protective effects and underlying mechanism are not fully identified. In this study, we attempted to determine whether pretreatment with KPOS induced significant barrier protective activities in lipopolysaccharide (LPS)-stimulated human umbilical vein endothelial cells (HUVECs).

Methods

The anti-inflammatory activities of KPOS were determined by measuring solute flux, neutrophil adhesion and migration and activation of pro-inflammatory proteins in LPS-activated HUVECs.

Results

We found that KPOS inhibited LPS-induced barrier disruption, expression of cell adhesion molecules, neutrophil adhesion and transendothelial migration of neutrophils to HUVECs. Further studies revealed that KPOS suppressed the production of tumor necrosis factor-α (TNF-α) and activation of nuclear factor-κB (NF-κB) by LPS, and that anti-inflammatory activities of KPOS were better than those of KP.

Conclusion

Collectively, these results suggest that KPOS possesses barrier integrity activity, inhibitory activity on cell adhesion and migration to endothelial cells by blocking the activation of NF-κB expression and production of TNF-α, thereby endorsing its usefulness as therapy for vascular inflammatory diseases.

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Abbreviations

KP:

Kaempferol

KPOS:

Kaempferol-3-O-sophoroside

LPS:

Lipopolysaccharide

CAM:

Cell adhesion molecule

VCAM:

Vascular cell adhesion molecule

ICAM:

Intracellular cell adhesion molecule

NF-κB:

Nuclear factor-κB

TNF:

Tumor necrosis factor

BDMC:

Bisdemethoxycurcumin

TEM:

Transendothelial migration

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Acknowledgments

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government [MEST] (No. 2011-003410, 2011-0026695, 2011-0030124).

Conflict of interest

The authors declare no conflicts of interest.

Author information

Authors and Affiliations

  1. Department of Herbal Medicinal Pharmacology, College of Herbal Bio-Industry, Daegu Haany University, Gyeongsan, 712-715, Republic of Korea

    Tae Hoon Kim

  2. Department of Anatomy and Histology, College of Oriental Medicine, Daegu Haany University, Gyeongsan, 712-715, Republic of Korea

    Sae-Kwang Ku

  3. Senior Industry Cluster Agency, Youngdong University, Youngdong, 370-701, Republic of Korea

    In-Chul Lee

  4. College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-gu, Daegu, 702-701, Republic of Korea

    Jong-Sup Bae

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  1. Tae Hoon Kim
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Corresponding author

Correspondence to Jong-Sup Bae.

Additional information

Responsible Editor: Liwu Li.

T. H. Kim, S.-K. Ku and I.-C. Lee contributed equally to this work.

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Kim, T.H., Ku, SK., Lee, IC. et al. Anti-inflammatory effects of kaempferol-3-O-sophoroside in human endothelial cells. Inflamm. Res. 61, 217–224 (2012). https://doi.org/10.1007/s00011-011-0403-9

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  • DOI: https://doi.org/10.1007/s00011-011-0403-9

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