Abstract
Uniform-sized amino-modified silica nanoparticles have been prepared by the controlled synchronous hydrolysis of tetraethoxysilane and N-(β-amimoethyl)-γ-aminopropyltriethoxysilane in water nanodroplet of the wa-ter-in-oil microemulsion. These nanoparticles display positive charge potential at definited pH. This is due to the presence of amino groups on the surface of the nanoparticles. Nanoparticles-plasmid DNA complexes can easily form through electrostatical binding between the positive charges of the amino-modified silica nanoparticles and the negative charges of the plasmid DNA. The complexes can be also dissociated under alkaline pH or high ionic strength conditions. And enzymatic digestion of the plasmid DNA is almost inhibited by these nanoparticles complexes. A novel non-viral gene carrier based on the amino-modified silica nanoparticles is proposed under the combination of nanotechnology, biotechnology and gene engineering technology. The plasmid DNA can successfully cross various systemic barriers to COS-7 cells as well as mediate high expression of Green Fluorescence Protein (GFP) gene in cells by use of this novel gene carrier.
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References
Boussif, O., Lezoualch, F., Zanta, M. A. et al., A versatile vector for gene and oligonucleotide transfer into cells in culture andin vivo: Polyethylenimine, Proc. Natl. Acad. Sci. USA, 1995, 92: 7297.
Reszka, R., Zhu, J. H., Weber, F. et al., Liposome mediated transfer of marker and cytokine genes into rat and human Glioblastoma cellsin vitro andin vivo, J. Liposome Res., 1995, 5: 149.
Singh, M., Briones, M., Ott, G. et al., Cationic microparticles: a potent delivery system for DNA vaccines, PNAS J, 2000, 97 (2): 811.
Aderson, W. F., Humane gene therapy, Nature, 1998, 392 (6679): 25.
Antimisiaris, S. G., Jayaseker, P., Gregoriadis, G., Liposomes as vaccine carriers, J. Immun. Meth., 1993, 166: 271.
Davis, S. S., Biomedical application of nanotechnology — implication for drug targeting and gene therapy, Trends in Biotech., 1997, 15: 217.
Bender, A. R., Briesen, H. Y., Kreuter, J. et al., Efficiencey of nanoparticles as a carrier system for antiviral agents in humane immunodeficiency virus-infected human monocytes/macrophagesin vitro, Antimicrobial Agents & Chemotherapy, 1996, 40 (6): 1467.
Luo, D., Saltzman, W. M., Enhancement of transfection by physical concentration of DNA at the cell surface, Nat. Biotech., 2000, 18: 893.
Junghans, M., Kreuter, J., Zimmer, A., Antisense delivery using protamine-oligonucleotide particles, Nucleic Acid Res., 2000, 28 (10): e45.
Schwab, G., Chavany, C., Duroux, I. et al., Antisense oligonucleotide adsorbed to polyalkylcyanoacrylate nanoparticles specifically inhibit mutated Ha-ras-mediated cell proliferation and tumorigenicity in nude mice, Proc. Natl. Acad. Sci. USA, 1994, 91: 10460.
Jagur-Grodzinski, J., Biomedical application of functional polymers, Reactive & Functional Polymers, 1999, 39: 99.
Zimmer, A., Antisense oligonucleotide delivery with polyhexylcyanoacrylate nanoparticles as carriers, A Companion to Methods in Enzymology, 1999, 18: 286.
Erbacher, P., Zou, S., Bettinger, T. et al., Chitosan-based vector/ DNA complexes for gene delivery, biophysical characteristics and transfection ability. Pharm Res, 1998, 15 (9): 1332.
Zhu, S. G., Lv, H. B., Xiang, Z. Z. et al., A novel no-viral gene carrier: poly-L-lysine silica nanoparticles, Chinese Science Bulletin, 2002, 47(8): 654.
Kneuer, C., Sameti, M., Haltner, E. G. et al., Silica nanoparticles modified with aminosilanes as carriers for plasmid DNA, Int. J. Pharm., 2000, 196 (2): 257.
Sandhu, K. K., McIntosh, C. M., Simard, J. M. et al., Gold nanoparticles-mediated transfection of mammalian cells, Bioconjug. Chem., 2002, 13 (1): 3.
He, Q., Mitchell, A. R., Johnson, S. L. et al., Calcium phosphate nanoparticles adjuvant, American Society for Microbiology, 2000, 7 (6): 899.
Kneuer, C., Sameti, M., Bakowsky, U. et al., 2A nonviral DNA delivery system based on surfaced modified silica-nanoparticles can efficiently transfect cellsin vitro, Bioconjug. Chem., 2000, 11 (6): 926.
He, X. X., Wang, K. M., Tan, W. H. et al., A novel fluorescent label based on biological fluorescent nanoparticles and its application in cell recognition, Chinese Science Bulletin, 2001, 46(23): 1962.
He, X. X., Wang, K. M., Tan, W. H. et al., Photostable luminescent nanoparticles as biological label for cell recognition of system lupus erythematosus (SLE) patients, J. Nanosci. Nanotech., 2002, 2(3): 317.
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He, X., Wang, K., Tan, W. et al. A novel gene carrier based on amino-modified silica nanoparticles. Chin.Sci.Bull. 48, 223–228 (2003). https://doi.org/10.1007/BF03183287
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DOI: https://doi.org/10.1007/BF03183287