Abstract
To find pharmacologically active components ofAgrocybe cylindracea, its basidiocarps were extracted with water. The extracts were separated by DEAE cellulose column chromatography, Sepharose CL-4B gel filtration, and Concanavalin A-Sepharose 4B affinity chromatography. Among the obtained fractions fromA. cylindracea, fraction IN which was the neutral proteinbound-polysaccharide fraction exhibited a marked antitumor activity and it was tentatively named “Cylindan”. It showed about 70% of tumor inhibition against the solid form of sarcoma 180 when a dose of 30 mg/kg/day was intraperitoneally injected into ICR mice. When each fraction was examined by chemical analysis, Cylindan consisted of 85% polysaccharide, 3% protein and 1% hexosamine. Its polysaccharide moiety contained glucose, mannose, fucose and galactose and its protein moiety contained the comparatively large amounts of aspartic acid and glycine, and other 11 amino acids.
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Hyun, J.W., Kim, C.K., Park, S.H. et al. Antitumor components ofAgrocybe cylindracea . Arch. Pharm. Res. 19, 207–212 (1996). https://doi.org/10.1007/BF02976891
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DOI: https://doi.org/10.1007/BF02976891