Skip to main content
SpringerLink
Log in

Laboratory-scale production of lignin-degrading enzymes by free and entrapped cells ofPhanerochœte chrysosporium in a tubular air-lift bioreactor

  • Papers
  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

The production of lignin-degrading enzymes by free and entrapped cells ofPhanerochœte chrysosporium in a tubular air-lift bioreactor was studied. Under optimized cultural conditions the production of lignin peroxidase by free cells, calcium-alginate-entrapped cells and scouring-mesh-entrapped cells was in a ratio of 520∶720∶950 mU/mL, while the production of manganese peroxidase was in a ratio of 350∶480∶620 mU/mL. The stability of the entrapped cells by fed-batch systems was highest after 3 feeding experiments which is similarly demonstrated in the repeated use of the preparations in batch system.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

LiP:

lignin peroxidase

MnP:

manganese peroxidase

References

  • Asada Y., Miyabe M., Kikkawa M., Kuwahara M.: An extracellular NADH-oxidising peroxidase produced by a lignindegrading basidiomycete,Phanerochœte chrysosporium.J. Ferment. Technol. 65, 483–487 (1987).

    Article  CAS  Google Scholar 

  • Asther M., Bellon-Fontaine M.N., Capdevila C., Corrieu G.: A thermodynamic model to predictPhanerochœte chrysosporium INA-12 adhesion to various solid carriers in relation to lignin peroxidase production.Biotechnol. Bioeng. 35, 477–482 (1990).

    Article  CAS  PubMed  Google Scholar 

  • Bajpai P.K., Wallace J.B., Maragaritis A.: Effect of calcium chloride concentration on ethanol production and growth of immobilizedZymononas mobilis.J. Ferment. Technol. 63, 199–203 (1985).

    CAS  Google Scholar 

  • Bonnarme P., Delattre M., Corrieu G., AstherM.: Peroxidase secretion by pellets or immobilized cells ofPhanerochœte chrysosporium SEM-F-1767 and INA-12 in relation to organelle content.Enzyme Microb. Technol. 13, 727–733 (1991).

    Article  CAS  Google Scholar 

  • Bonnarme M., Delattre H., Drouet G., Corrieu G., Asther M.: Toward a control of lignin and manganese peroxidase hypersecretion byPhanerochœte chrysosporium in agitated vessels: Evidence of the superiority of pneumatic bioreactors on machanically agitated bioreactors.Biotechnol. Bioeng. 41, 440–450 (1993).

    Article  CAS  PubMed  Google Scholar 

  • Chen A.H.C., Dosoretz C.G., Grethlein H.E.: Ligninase production by immobilised cultures ofPhanerochœte chrysosporium grown under nitrogen-sufficient conditions.Enzyme Microb. Technol. 13, 40–407 (1991).

    Article  Google Scholar 

  • Cornwell K.L., Tinland-Butez M.F., Tar Done P.J., Cabasso I., Hammel K.E.: Lignin degradation and lignin peroxidase production in cultures ofPhanerochœte chrysosporium immobilized on porous ceramic supports.Enzyme Microb. Technol. 12, 916–920 (1990).

    Article  CAS  Google Scholar 

  • Darah I., Ibrahim C.O.: Production of lignin peroxidase and managnese peroxidase byPhanerochate chrysosporium in submerged cultures.Asia Pac. J. Mol. Biol. Biotechnol. 4, 154–167 (1996a).

    Google Scholar 

  • Darah I., Ibrahim C.O.: Effect of agitation on lignin-degrading enzyme production byPhanerochœte chrysosporium grown in shake-flask cultures.Asia. Pac. J. Mol. Biol. Biotechnol. 4, 174–182 (1996b).

    Google Scholar 

  • Darah I., Ibrahim C.O.: Correlation between degradation of lignin degrading enzymes and proteolytic activities in submerged cultures ofPhanerochœte chrysosporium.Asia Pac. J. Mol. Biol. Biotechnol. 4, 203–209 (1996c).

    Google Scholar 

  • Fujimura M., Kato J., Tosa T., Chibata I.: Continous production ofl-arginine using immobilized growingSerratia marcescens cells: Effectiveness of supply of oxygen gas.Appl. Microbiol. Biotechnol. 19, 79–84 (1984).

    Article  CAS  Google Scholar 

  • Gamara J.A., Cuevas C.M., Lescao G.: Production of ethanol by a stirred catalytic basket reactor with immobilized yeast cells.J. Ferment. Technol. 64, 25–28 (1986).

    Article  Google Scholar 

  • Glen J.K., Gold M.H.: Purification and characterization of an extracellular Mn(II)-dependent peroxidase from the lignin-degrading basidiomycete,Phanerochœte chrysosporium.Arch. Biochem. Biophys. 242, 329–341 (1985).

    Article  Google Scholar 

  • Grosmann B., Rehm H.J.: Oxygen uptake of microorganisms entrapped in calcium alginate.Appl. Microb. Biotechnol. 23, 163–167 (1986).

    Google Scholar 

  • Heck J., Onken U.: Hysteresis effects in suspended solid particles in bubble columns with and without draft tube.Chem. Eng. Sci. 42, 121–1212 (1987).

    Google Scholar 

  • Janshekar H., Fiechter A.: Cultivation ofPhanerochœte chrysosporium and production of lignin peroxidases in submerged stirred tank reactors.J. Biotechnol. 8, 97–112 (1988).

    Article  CAS  Google Scholar 

  • Kiese S., Elaner H.G., Onken U.: A simple laboratory air-lift fermentor.Biotechnol. Lett. 2, 345–350 (1980).

    Article  Google Scholar 

  • Kirk T.K., Croan S., Tien M., Murtagh K.E., Farrell R.L.: Production of a multiple ligninase byPhanerochœte chrysosporium: effect of selected growth conditions and use of a mutant strain.Enzyme Microb. Technol. 8, 27–32 (1986).

    Article  CAS  Google Scholar 

  • Leisola M.S.A., Fiechter A.: Ligninase production in agitated conditions byPhanerochœte chrysosporium.FEMS Microbiol. Lett. 29, 33–36 (1985).

    Article  CAS  Google Scholar 

  • Leisola M.S.A., Thanei-Wyss U., Fiechter A.: Strategies for production of high ligninase activities byPhanerochœte chrysosporium.J. Biotechnol. 3, 97–107 (1985).

    Article  CAS  Google Scholar 

  • Linko S., Zhong L.C.: Comparison of different carrier methods of immobilization for lignin peroxidase production byPhanerochœte chrysosporium.Bioproc. Eng. 6, 43–48 (1987).

    Article  Google Scholar 

  • Linko S.: Continous production of lignin peroxidase by immobilizedPhanerochœte chrysosporium in a pilot scale bioreactor.J. Biotechnol. 8, 163–170 (1988).

    Article  CAS  Google Scholar 

  • Longo M.A., Novella I.S., Garcia A., Diaz M.: Diffusion of proteases in calcium alginate beadsEnzyme Microb. Technol. 14, 586–590 (1992).

    Article  PubMed  CAS  Google Scholar 

  • Michel F.C., Grulke E.A., Reddy C.A.: Development of a stirred tank reactor system for the production of lignin peroxidases (ligninases) byPhanerochœte chrysosporium BKM-F-1767.J. Industr. Microbiol. 5, 103–112 (1990).

    Article  CAS  Google Scholar 

  • Noor Izani N.J., Ibrahim C.O.: Lipase production using immobilizedAspergillus niger.J. Biosci. 3, 31–41 (1992).

    Google Scholar 

  • Paszczynski A., Huynh V.B., Crawford R.: Enzymatic activities of an manganese-dependent peroxidase fromPhanerochœte chrysosporium.FEMS Microbiol. Lett. 29, 37–41 (1985).

    Article  CAS  Google Scholar 

  • Ruckenstein E., Wang X.B.: Production of lignin peroxidase byPhanerochœte chrysosporium immobilized on porous poly-(styrene-divinylbenzene) carrier and its application to the degrading of 2-chlorophenol.Biotechnol. Bioeng. 44, 79–86 (1994).

    Article  CAS  PubMed  Google Scholar 

  • Sandhukan R., Roy S.K., Chakrabarty S.L.: Immobilization of α-amylase fromMyceliophthora thermophila D-14 (ATCC 48104).Enzyme Microb. Technol. 15, 801–804 (1993).

    Article  Google Scholar 

  • Schmidt B., Heimgartner U., Kozulic B., Leisola M.S.A.: Lignin peroxidases are oligomannose type glycoproteins.J. Biotechnol. 13, 223–228 (1990).

    Article  CAS  Google Scholar 

  • Somogyi-Nelson M.: Notes on sugar determination.J. Biol. Chem. 195, 19–23 (1952).

    Google Scholar 

  • Tanaka H., Irie S., Ochi H.: A novel immobilization method for prevention of cell leakage from the gel matrix.J. Ferment. Bioeng. 68, 216–219 (1989).

    Article  CAS  Google Scholar 

  • Tien M., Kirk T.K.: Lignin peroxidase ofPhanerochœte chrysosporium.Methods Enzymol. 161, 238–249 (1988).

    Article  CAS  Google Scholar 

  • Tonon F., Odier E.: Influence of veratryl alcohol and hydrogen peroxide on ligninase activity and ligninase production byPhanerochœte chrysosporium.Appl. Environ. Microbiol. 54, 466–472 (1988).

    PubMed  CAS  Google Scholar 

  • Trager M., Qazi G.N., Onken U., Chopra C.L.: Comparison of air-lift and stirred reactors for fermentation withAspergillus niger.J. Ferment. Bioeng. 68, 112–116 (1989).

    Article  Google Scholar 

  • Willlenrschausen H., Jäger A., Graf H.: Ligninase production ofPhanerochœte chrysosporium by immobilization in bioreactors.J. Biotechnol. 6, 239–243 (1987).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Fermentation and Enzyme Technology Laboratory, School of Biological Sciences, University Sains Malaysia, 11800 Minden, Penang, Malaysia

    I. Darah & C. O. Ibrahim

Authors
  1. I. Darah
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. O. Ibrahim
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Darah, I., Ibrahim, C.O. Laboratory-scale production of lignin-degrading enzymes by free and entrapped cells ofPhanerochœte chrysosporium in a tubular air-lift bioreactor. Folia Microbiol 43, 161–168 (1998). https://doi.org/10.1007/BF02816503

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02816503

Keywords

Search

Navigation