Skip to main content
SpringerLink
Log in

Genetic evidence supports the new anatolian lupine accession,Lupinus anatolicus, as an old world “rough-seeded” lupine (sectionScabrispermae) related toL. pilosus

  • Published:
Folia Geobotanica Aims and scope Submit manuscript

Abstract

A noteworthy wild lupine accession was recently discovered in southwestern Turkey and was proposed as a new separate Old World “smooth-seeded” species close toL. micranthus and namedL. anatolicus. Its species status was controversial with respect to cytological and crossability data. In order to examine the position and the evolutionary relationships of this Anatolian accession relative to the Old World lupines, we investigated new data from seed coat micromorphology, and from internal transcribed spacer (ITS) nucleotide sequences of the nuclear ribosomal DNA repeat. The micromorphological seed coat pattern ofL. anatolicus, as revealed by scanning electron microscopy, is characterized by pluricellular tubercles, which represent the typical and unique pattern of the Old World “rough-seeded” lupines (sect.Scabrispermae). In accordance with the micromorphological results, the genetic distances and phylogenetic relationships among the Old World lupines, estimated from ITS data, unambiguously support the new Anatolian lupine accession as part of theL. pilosus-L. palaestinus lineage within the strongly monophyletic group containing all theScabrispermae. The results provided in this study, together with other lines of data available from the literature, are thus hardly compatible with the hypothesis that this new Anatolian lupine accession could be related to Old World “smooth-seeded” lupines (includingL. micranthus); instead, it appears closely related toL. pilosus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aïnouche A. (1991):Variabilités morphologiques et biochimiques des graines de populations du genre LupinusL. (Papilionoideae)en Algérie. Magister Thesis (n. 08/91M/ISN), Université des Sciences et Techniques d’Alger, Alger.

    Google Scholar 

  • Aïnouche A. (1998):Diversité et évolution du genre Lupinus (Fabaceae). PhD Thesis, Université de Rennes-1, Rennes.

    Google Scholar 

  • Aïnouche A. &Bayer R.J. (1999a): Phylogenetic relationships inLupinus (Fabaceae: Papilionoideae) based on internal transcribed spacer sequences (ITS) of nuclear ribosomal DNA.Amer. J. Bot. 86: 590–607.

    Article  Google Scholar 

  • Aïnouche A. &Bayer R.J. (1999b): Phylogenetic relationships among and within the Old and New WorldLupinus species (Fabaceae) based on internal transcribed spacer sequences of nuclear ribosomal DNA. In:Hill G.D. (ed.),Proceedings of the Eighth international lupin conference, May 11–16 1996, California (USA), International Lupin Association, Lincoln University, Canterbury, pp. 236–244.

    Google Scholar 

  • Aïnouche A., Greinwald R., Witte L. &Huon A. (1996): Seed alkaloid composition ofLupinus tassilicus Maire (Fabaceae: Genisteae) and comparison with its related rough seeded lupin species.Biochem. Syst. Ecol. 24: 405–414.

    Article  Google Scholar 

  • Badr A., Martin W. &Jensen J. (1994): Chloroplast DNA restriction polymorphism inGenisteae (Leguminosae) suggests a common origin for European and American lupinesPl. Syst. Evol. 193: 95–106.

    Article  CAS  Google Scholar 

  • Bayer R.J, Hufford L. &Soltis D.E. (1996): Phylogenetic relationships inSarraceniaceae based onrbcL and ITS sequences.Syst. Bot. 21: 121–134.

    Article  Google Scholar 

  • Belteky B., Kovacs I. &Desbrosses P. (1983):Le Lupin — Histoire et utilisation de l’une des Légumineuses les plus riches du monde en protéines. UNAPEL, Mairie de Millançay, Romorantin.

    Google Scholar 

  • Bragg L.H. (1983): Seed coats of someLupinus species.Scan. Electron Microscop. 4: 1739–1745.

    Google Scholar 

  • Carstairs S.A., Buirchell B.J. &Cowling W.A. (1992): Chromosome number, size and intercrossing ability of three Old World lupines,Lupinus princei Harms,L. atlanticus Gladstones andL. digitatus Forsskal, and implications for cyto-systematic relationships among the rough-seeded lupins.J. Roy. Soc. W. Australia 75: 83–88.

    Google Scholar 

  • Clements J.C., Buirchell B.J. &Cowling W.A. (1996): Relationship between morphological variation and geographical origin or selection history inLupinus pilosus.Pl. Breed. 115: 16–22.

    Article  Google Scholar 

  • Corner U.J.H. (1951): The leguminous seed.Phytomorphology 1: 117–150.

    Google Scholar 

  • Cristofolini G. (1989): A serological contribution to the systematics of the genusLupinus (Fabaceae).Pl. Syst. Evol. 166: 265–278.

    Article  Google Scholar 

  • Davis P.H. (1970):Flora of Turkey and the Aegaean Islands 3. Edinburgh University Press, Edinburgh.

    Google Scholar 

  • Doyle J.J. (1995): DNA data and legume phylogeny: a progress report. In:Crisp M. &Doyle J.J. (eds.),Advances in legume systematics 7, Phylogeny, Royal Botanic Gardens, Kew, pp. 11–30.

    Google Scholar 

  • Doyle J.J. &Doyle J.L. (1987): A rapid DNA isolation procedure for small quantities of fresh leaf tissue.Phytochem. Bull. 19: 11–15.

    Google Scholar 

  • Felsenstein J. (1985): Confidence limits on phylogenies: an approach using the bootstrap.Evolution 39: 783–791.

    Article  Google Scholar 

  • Gladstones J.S. (1974): Lupins of the Mediterranean region and Africa.W. Austral. Dept. Agric. Tech. Bull. 26: 1–48.

    Google Scholar 

  • Gladstones J.S. (1981): Recent developments in the understanding, improvement and use ofLupinus. In:Summerfield R.J. &Bunting A.H. (eds.),Advances in legume science, Royal Botanic Gardens, Kew, pp. 603–611.

    Google Scholar 

  • Gladstones J.S. (1984): Present situation and potential of mediterranean-african lupins for crop production. In:International Lupin Association (ed.),Proceedings of the third International lupin conference, June 4–8, 1984, La Rochelle (France), SPIT, Evry, pp. 17–37.

    Google Scholar 

  • Gladstones J.S. &Crosbie G.B. (1979): Lupin wild types introduced into western Australia to 1973.W. Austral. Dept. Agric. Tech. Bull. 43: 1–44.

    Google Scholar 

  • Gunn C.R. (1981): Seed ofLeguminosae. In:Polhill R.M. &Raven P.H. (eds.),Advances in legume systematics 1, Royal Botanic Gardens, Kew, pp. 913–926.

    Google Scholar 

  • Gupta S., Buirchell B.J. &Cowling W.A. (1996): Interspecific reproductive barriers and genomic similarity among the rough-seededLupinus species.Pl. Breed. 115: 123–127.

    Article  Google Scholar 

  • Heyn C.C. &Herrnstadt I. (1977): Seed coat structure of old worldLupinus species.Bot. Not. 130: 427–435.

    Google Scholar 

  • Käss E. &Wink M. (1997): Molecular phylogeny and phylogeography ofLupinus (Leguminosae) inferred from nucleotide sequences of the rbcL gene and ITS 1 + 2 regions of rDNA.Pl. Syst. Evol. 208: 139–167.

    Article  Google Scholar 

  • Kazimierski T. (1961): Interspecific hybridization ofLupinus.Genet. Polon. 2: 97–102.

    Google Scholar 

  • Kazimierski T. (1988): An attempt to present lupin evolution of the old world. Materials and impressions. In:Twardowski T. (ed.),Proceedings of the fifth International lupin conference, July 5–8, 1988, PWRiL, Poznań, pp. 103–109.

    Google Scholar 

  • Lersten N.R. (1981): Testa topography inLeguminosae, subfamilyPapilionoideae.Proc. Iowa Acad. Sci. 88: 181–191.

    Google Scholar 

  • Monteiro R. (1987): Seed testa pattern of unifoliolate species ofLupinus L. (Leguminosae).Salusvita 6: 20–31.

    Google Scholar 

  • Nowacki E. (1963): Inheritance and biosynthesis of alkaloids in lupin.Genet. Polon. 4: 161–202.

    Google Scholar 

  • Obermayer R., Greilhuber J. &Swiecicki W.K. (1999): Flow cytometric determination of genome size in some Old WorldLupinus species.Pl. Biol. 1: 403–407.

    Google Scholar 

  • Pazy B., Plittmann U. &Heyn C.C. (1981): Genetic relationships betweenLupinus pilosus andL. palaestinus (Fabaceae).Pl. Syst. Evol. 137: 39–44.

    Article  Google Scholar 

  • Plitmann U. &Heyn C.C. (1984): Old WorldLupinus: Taxonomy, evolutionary relationships and links with New World species. In:International Lupin Association (ed.),Proceedings of the third International lupin conference, June 4–8, 1984, La Rochelle, France, SPIT, Evry, pp. 55–66.

    Google Scholar 

  • Plitmann U. &Pazy B. (1984): Cytogeographical distribution of old worldLupinus.Webbia 38: 531–539.

    Google Scholar 

  • Plitmann U., Heyn C.C. &Pazy B. (1980): Biological flora of Israel. 7.Lupinus palaestinus Boiss. andL. pilosus Murr.Israel J. Bot. 28: 108–130.

    Google Scholar 

  • Przybylska J. &Zimniak-Przybylska Z. (1995): Electrophoretic patterns of seed globulins in the Old-WorldLupinus species.Genet. Res. Crop Evol. 42: 69–75.

    Article  Google Scholar 

  • Roy N.N. &Gladstones J.S. (1988): Further studies with interspecific hybridization among Mediterranean/African lupin species.Theor. Appl. Genet. 75: 606–609.

    Article  Google Scholar 

  • Saint-Martin M. (1986): Micromorphologie tégumentaire des graines desPapilionaceae.Bull. Soc. Bot. France, Lett. Bot. 133: 137–153.

    Google Scholar 

  • Salmanowicz B.P. &Przybylska J. (1994): Electrophoretic patterns of seed albumins in the Old-WorldLupinus species (Fabaceae): variation in the 2S albumin class.Pl. Syst. Evol. 192: 67–78.

    Article  CAS  Google Scholar 

  • Sanger F., Niklen S. &Coulson A.R. (1977): DNA sequencing with chain-terminating inhibitors.Proc. Natl. Acad. Sci. U.S.A. 74: 5463–5467.

    Article  PubMed  CAS  Google Scholar 

  • Serrato Valenti G., Melone M., Ferro M. &Bozzini A. (1989): Comparative studies on testa structure of “hard-seeded” and “soft-seeded” varieties ofLupinus angustifolius L. (Leguminosae) and on mechanisms of water entry.Seed Sci. Technol. 17: 563–581.

    Google Scholar 

  • Swiecicki W. (1988): Lupin gene resources in the Old World. In:Twardowski T. (ed.),Proceedings of the fifth International Lupin Conference, July 5–8, 1988, PWRiL, Poznań, pp. 2–14.

  • Swiecicki W., Swiecicki W. K. &Wolko B. (1996):Lupinus anatolicus — a new lupin species of the Old World.Genet. Res. Crop Evol. 43: 109–117.

    Article  Google Scholar 

  • Swiecicki W., Wolko B. & Jach K. (1994):Lupinus anatolicus — an unknown species of the Old World. In:Neves-Martins J.M. & Beirao da Costa M.L. (eds.),Advances in lupin research, Proceedings of the Seventh International Lupin Conference, Evora (Portugal), April 18–23, 1993, I.S. de Agronomia (ISA Press), Lisboa, pp. 63–64.

  • Swofford D.L. (1993): PAUP:Phylogenetic analysis using parsimony, Version 3.3.1. Illinois Natural History Survey, Champaign.

    Google Scholar 

  • White T.J., Bruns Y., Lee S. &Taylor J. (1990): Amplification and direct sequencing of fungal RNA genes for phylogenetics. In:Innis M., Gelfand D., Sninsky J. &White T. (eds.),pcr protocols: a guide to methods and applications, Academic Press, San Diego, pp. 315–322.

    Google Scholar 

  • Wink M., Meibner C. &Witte L. (1995): Patterns of quinolizidine alkaloids in 56 species of the genusLupinus.Phytochemistry 38: 139–153.

    Article  CAS  Google Scholar 

  • Wolko B. &Weeden N.F. (1990): Isozyme number as an indicator of phylogeny inLupinus.Genet. Polon. 31: 179–187.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Service de Biosystématique et Génétique des Populations Végétales, UMR CNRS Ecobio 6553, Université de Rennes-1, Campus Scientifique de Beaulieu, Bâtiment 14, F-35042, Rennes, Cedex, France

    Abdelkader Aïnouche

  2. CSIRO, Plant Industry, Molecular Systematics Laboratory, Australian National Herbarium, GPO Box 1600, 2601, Canberra, ACT, Australia

    Randall James Bayer

Authors
  1. Abdelkader Aïnouche
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Randall James Bayer
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Abdelkader Aïnouche.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Aïnouche, A., Bayer, R.J. Genetic evidence supports the new anatolian lupine accession,Lupinus anatolicus, as an old world “rough-seeded” lupine (sectionScabrispermae) related toL. pilosus . Folia Geobot 35, 83–95 (2000). https://doi.org/10.1007/BF02803088

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02803088

Keywords

Search

Navigation