Skip to main content
SpringerLink
Log in

The nuclease activity of the endogenous differentiation factor of the HL-60 cell line

  • Published:
Russian Journal of Bioorganic Chemistry Aims and scope Submit manuscript

Abstract

A structural homology between the endogenous differentiation factor of the HL-60 cell line of promyelocyte leukemia (HLDF) and several DNA/RNA-binding and DNA/RNA-hydrolyzing proteins was revealed, and expression of thehldf gene in prokaryotic systems was studied. On the basis of these experiments, the amino acid sequence of an 8-membered fragment of HLDF with potential nuclease activity was identified. The synthetic octapeptide RRWHRLKE was shown to be capable of the cleavage of RNA, linear DNA from phage λ, and all forms of plasmid DNA. We established that treatment of the HL-60 cell culture with this peptide (10−6 M) results in an increase in the number of apoptotic cells and suggested that HLDF is involved in processes of apoptosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

C2-Cer:

N′-acetylsphingosine

DNS:

dansyl

DOPA:

3,4-dihydroxyphenylalanine

DTE:

1,4-dithioerythritol

HEPES:

N-2-hydroxyethylpiperazine-N′-ethanesulfonic acid

HLDF:

human leukemia differentiation factor from the LH-60 cell line

NBT:

Nitro Blue Tetrazolium

PBS:

phosphate buffered saline (isotonic sodium/potassium phosphate buffer)

PITC:

phenyl isothiocyanate

References

  1. Witter, C.U., Bauw, G., and Krokan, H.,Biochemistry, 1989, vol. 28, pp. 780–784.

    Article  Google Scholar 

  2. Shaper, N.L., Grafstrom, R.H., and Grossman, L.,J. Biol. Chem., 1982, vol. 257, pp. 13455–13458.

    PubMed  CAS  Google Scholar 

  3. Hang, H., Chenna, A., Fraenkel-Conrat, H., and Singer, B.,Proc. Natl. Acad. Sci. USA, 1996, vol. 93, pp. 13737–13741.

    Article  PubMed  CAS  Google Scholar 

  4. Strauss, P. and Holt, C.,J. Biol. Chem., 1998, vol. 273, pp. 14435–14441.

    Article  PubMed  CAS  Google Scholar 

  5. Vlassov, V.A., Dymshits, G.M., and Lavrik, O.I.,Mol. Biol. (Moscow), 1998, vol. 32, pp. 5–18.

    Google Scholar 

  6. Steitz, T.A.,J. Biol. Chem., 1999, vol. 274, pp. 17395–17398.

    Article  PubMed  CAS  Google Scholar 

  7. Kostrewa, D. and Winkler, F.,Biochemistry, 1995, vol. 34, pp. 683–696.

    Article  PubMed  CAS  Google Scholar 

  8. Perona, J. and Martin, A.,J. Mol. Biol., 1997, vol. 273, pp. 207–225.

    Article  PubMed  CAS  Google Scholar 

  9. Newman, M., Strzelecka, T., Dorner, L., Schildkraut, I., and Aggarwal, A.,Science, 1995, vol. 269, pp. 656–663.

    Article  PubMed  CAS  Google Scholar 

  10. Pierre, J. and Laval, J.,J. Biol. Chem., 1981, vol. 256, pp. 10217–10220.

    PubMed  CAS  Google Scholar 

  11. Barbier, B. and Brack, A.,J. Am. Chem. Soc., 1992, vol. 114, pp. 3511–3515.

    Article  CAS  Google Scholar 

  12. Vlassov, V.V., Sil’nikov, V.N., and Zenkova, M.A.,Mol. Biol. (Moscow), 1998, vol. 32, pp. 62–71.

    Google Scholar 

  13. Podyminogin, M., Vlassov, V., and Giege, R.,Nucleic Acids Res., 1993, vol. 21, pp. 5950–5956.

    Article  PubMed  CAS  Google Scholar 

  14. Pyshnyi, D.V., Repkova, M.N., Lokhov, S.G., Ivanova, E.M., Ven’yaminova, A.G., and Zarytova, V.F.,Bioorg. Khim., 1997, vol. 23, pp. 497–504.

    CAS  Google Scholar 

  15. Truffert, J.-C. and Asseline, U.,Tetrahedron, 1996, vol. 52, pp. 3005–3016.

    Article  CAS  Google Scholar 

  16. Harford, K. and Sarcar, B.,Biochemistry, 1996, vol. 35, pp. 4271–4278.

    Article  PubMed  CAS  Google Scholar 

  17. Morier-Teissier, F., Bernier, J., Lohez, M., and Catteau, J.,Anti-Cancer Drug Des., 1990, vol. 5, pp. 291–305.

    CAS  Google Scholar 

  18. Kostanyan, I.A., Astapova, M.I., Starovoitova, E.V., Dranitsyna, S.M., and Lipkin, V.M.,Bioorg. Khim., 1995, vol. 21, pp. 243–248.

    CAS  Google Scholar 

  19. Bhat, K. and Morrison, S.,Nucleic Acids Res., 1992, vol. 21, p. 2939.

    Article  Google Scholar 

  20. Kutuzov, M.A., Shmukler, B.E., Suslov, O.N., Zargarov, A.A., and Abdulaev, N.G.,Bioorg. Khim., 1992, vol. 18, pp. 623–634.

    PubMed  CAS  Google Scholar 

  21. Smirnova, E., Merkulova, M., Rakitina, T., Astapova, M., and Kostanyan, I., Abstracts of Papers,Second Advanced Course of Gene Therapy, Venice, Italy, 1997, p. 42.

  22. Burbelo, P.D., Utani, A., Pan, Z., and Yamada, Y.,Proc. Natl. Acad. Sci. USA, 1993, vol. 90, pp. 11543–11547.

    Article  PubMed  CAS  Google Scholar 

  23. Lukow, B., Bunz, F., Stillman, B., Lichter, P., and Schuetz, G.,Mol. Cell. Biol., 1994, vol. 14, pp. 1626–1634.

    Google Scholar 

  24. Stroumbakis, N., Li, Z., and Tolias, P.,Gene, 1994, vol. 143, pp. 171–177.

    Article  PubMed  CAS  Google Scholar 

  25. Tiranti, V., Rocchi, M., DiDonato, S., and Zeviani, M.,Gene, 1993, vol. 126, pp. 219–225.

    Article  PubMed  CAS  Google Scholar 

  26. Gerschenson, M., Houmiel, K., and Low, R.,Nucleic Acids Res., 1995, vol. 23, pp. 88–97.

    Article  PubMed  CAS  Google Scholar 

  27. Windsor, W., Syto, R., Tsarbopoulos, A., Zhang, R., Durkin, J., Baldwin, S., Paliwal, S., Mui, P., Pramanik, B., and Trotta, P.,Biochemistry, 1993, vol. 32, pp. 8807–8815.

    Article  PubMed  CAS  Google Scholar 

  28. Enari, M., Sakahira, H., Yokoyma, H., Okawa, K., Iwamatsu, A., and Nagata, S.,Nature, 1998, vol. 391, pp. 43–50.

    Article  PubMed  CAS  Google Scholar 

  29. Enari, M., Sakahira, H., Yokoyma, H., Okawa, K., Iwamatsu, A., and Nagata, S.,Nature, 1998, vol. 393, p. 256.

    Article  Google Scholar 

  30. Masuda, Y., Bennett, R., and Demple, B.,J. Biol. Chem., 1998, vol. 273, pp. 30360–30365.

    Article  PubMed  CAS  Google Scholar 

  31. Nachum, J. and Hart, D.,Biochem. Biophys. Res. Commun., 1982, vol. 105, pp. 1433–1439.

    Article  Google Scholar 

  32. Gimble, F., Duan, X., Hu, D., and Quiocho, F.,J. Biol. Chem., 1998, vol. 273, pp. 30524–30529.

    Article  PubMed  CAS  Google Scholar 

  33. Horton, N., Newberry, K., and Perona, J.,Proc. Natl. Acad. Sci. USA, 1998, vol. 95, pp. 13489–13494.

    Article  PubMed  CAS  Google Scholar 

  34. Halliwell, B. and Gutteridge, J.M.C.,J.Lab.Clin.Med., 1992, vol. 119, pp. 598–620.

    PubMed  CAS  Google Scholar 

  35. Flores, I., Martinez, A., Hennun, Y., and Myrida, I.,J. Immunol., 1998, vol. 160, pp. 3528–3533.

    PubMed  CAS  Google Scholar 

  36. Jia, L., Dourmashkin, R., Allen, P., Gray, A., Newland, A., and Kelsey, S.,Br. J. Haematol., 1997, vol. 98, pp. 673–685.

    Article  PubMed  CAS  Google Scholar 

  37. Naora, H., Nishida, T., Shindo, Y., Adachi, M., and Naora, H.,Biochem. Biophys. Res. Commun., 1996, vol. 5, pp. 258–264.

    Article  Google Scholar 

  38. Suzuki, A., Tsutomi, Y., Yamamoto, N., Shibutani, T., and Akhahane, K.,Mol. Cell. Biol., 1999, vol. 19, pp. 3842–3847.

    PubMed  CAS  Google Scholar 

  39. Gamen, S., Hanson, D.A., Kaspar, A., Naval, J., Krensky, A.M., and Anel, A.,J. Immunol., 1998, vol. 161, pp. 1758–1764.

    PubMed  CAS  Google Scholar 

  40. Kozyr, A., Kolesnikov, A., Alexandrova, E., Sashenko, L., Gnuchev, N., Favorov, P., and Gabibov, A.,Biotecnol. Appl. Biochem., 1999 (in press).

  41. Harlow, E. and Lane, D.,Antibodies: a Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory, 1988.

    Google Scholar 

  42. Levina, N.B., Slepak, V.Z., Kiselev, O.G., and Shemyakin, V.V.,Bioorg. Khim., 1989, vol. 5, pp. 24–31.

    Google Scholar 

  43. Onozaki, K., Urawa, H., Tamatani, T., Iwamuta, Y., Hashimoto, T., Baba, T., Suzuki, H., and Matsushima, K.,J. Immunol., 1988, vol. 140, pp. 112–119.

    PubMed  CAS  Google Scholar 

  44. Rodionov, I., Baru, M., and Ivanov, V.,Pept. Res., 1992, vol. 5, pp. 119–125.

    PubMed  CAS  Google Scholar 

  45. Maniatis, T., Fritsch, E., and Sambrook, J.,Molecular Cloning: a Laboratory Manual, Cold Spring Harbor, New York: Cold Spring Harbor Laboratory, 1982.

    Google Scholar 

  46. Gray, R.,Methods Enzymol., 1967, vol. 11, pp. 138–151.

    Google Scholar 

  47. Hartley, B.,Biochem. J., 1970, vol. 119, pp. 805–822.

    PubMed  CAS  Google Scholar 

  48. Hsu, S.M., Raine, L., and Fanger, H.,J. Histochem. Cytochem., 1981, vol. 25, pp. 577–589.

    Google Scholar 

  49. Telford, W.G., King, L.E., and Fraker, P.J.,J. Immunol. Methods, 1994, vol. 172, pp. 1–16.

    Article  PubMed  CAS  Google Scholar 

  50. Molotkovskaya, I.M., Gudima, G.O., Lyashenko, V.A., Molotkovsky, Yul.G., and Bergel’son, L.D.,Biol. Membr., 1989, vol. 2, pp. 499–506.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117871, Moscow, GSP-7, Russia

    S. M. Dranitsyna, I. A. Kostanyan, S. G. Andreeva, M. V. Astapova, O. V. Baeva, A. P. Bogachuk, I. M. Molotkovskaya, E. V. Smirnova & V. M. Lipkin

  2. University of Peoples’ Friendship, ul. Miklukho-Maklaya 6, 117198, Moscow, Russia

    I. I. Babichenko

  3. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (Pushchino Branch), Russian Academy of Sciences, 142292, Pushchino, Moscow oblast, Russia

    I. L. Rodionov

Authors
  1. S. M. Dranitsyna
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. A. Kostanyan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. G. Andreeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. V. Astapova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. I. I. Babichenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. O. V. Baeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. P. Bogachuk
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. I. M. Molotkovskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. I. L. Rodionov
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. E. V. Smirnova
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. V. M. Lipkin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to I. A. Kostanyan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dranitsyna, S.M., Kostanyan, I.A., Andreeva, S.G. et al. The nuclease activity of the endogenous differentiation factor of the HL-60 cell line. Russ J Bioorg Chem 26, 306–316 (2000). https://doi.org/10.1007/BF02759282

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02759282

Key words

Search

Navigation