Skip to main content
SpringerLink
Log in

A further study on the regulation of cyclic nucleotide phosphodiesterase activity in neuroblastoma cells: Effect of growth

  • Published:
In Vitro Aims and scope Submit manuscript

Summary

Adenosine 3′,5′-cyclic monophosphate (cyclic AMP) phosphodiesterase activity in mouse neuroblastoma cells in culture markedly increased during exponential growth and reached a maximal level at confluency; whereas guanosine 3′, 5′-cyclic monophosphate (cyclic GMP) phosphodiesterase activity only slightly but significantly increased under a similar experimental condition. The increase in cyclic AMP phosphodiesterase activity was blocked by both cycloheximide and dactinomycin, whereas the increase in cyclic GMP phosphodiesterase was blocked by only cycloheximide. When the confluent cells were replated at low density, the cyclic nucleotide phosphodiesterase activity decreased; however, when they were plated at high cell density which equaled confluency, the enzyme activity did not decrease. Unlike cyclic AMP phosphodiesterase activity, cyclic GMP phosphodiesterase activity did not change significantly in prostaglandin E1-treated cells, but decreased in cells treated with the inhibitor of phosphodiesterase. Like cyclic AMP phosphodiesterase activity, cyclic GMP phosphodiesterase activity also did not change in cells treated with serum-free medium, X-irradiation, sodium butyrate and 6-thioguanine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Prasad, K. N., and S. Kumar. 1973. Cyclic 3′,5′-AMP phosphodiesterase activity during cyclic AMP-induced differentiation of neuroblastoma cells in culture. Proc. Soc. Exp. Biol. Med. 142: 406–409.

    PubMed  CAS  Google Scholar 

  2. D’Armiento, M., G. S. Johnson, and I. Pastan. 1972. Regulation of adenosine 3′,5′-cyclic monophosphate phosphodiesterase activity in fibroblasts by intracellular concentrations of cyclic adenosine monophosphate. Proc. Natl. Acad. Sci. U.S.A. 69: 425–429.

    Article  Google Scholar 

  3. Maganiello, V., and M. Vaughan. 1972. Prostaglandin E1 effects on adenosine 3′,5′-cyclic monophosphate concentration and phosphodiesterase activity in fibroblasts. Proc. Natl. Acad. Sci. U.S.A. 69: 269–273.

    Article  PubMed  CAS  Google Scholar 

  4. Lynch, T. J., E. A. Tallant, and W. Y. Cheung. 1975. Marked reduction of cyclic GMP phosphodiesterase activity in virally transformed mouse fibroblasts. 65: 1115–1122.

    CAS  Google Scholar 

  5. Schwartz, J. P., and J. U. Passonneau. 1974. Cyclic AMP mediated induction of the cyclic AMP phosphodiesterase of C-6 glioma cells. Proc. Natl. Acad. Sci. U.S.A. 71: 3844–3848.

    Article  PubMed  CAS  Google Scholar 

  6. Schwartz, J. P., N. R. Morris, and B. L. Breckenridge. 1973. Adenosine 3′,5′-monophosphate in glial tumor cells. Alteration by 5-bromodeoxyuridine. J. Biol. Chem. 248: 2699–2704.

    PubMed  CAS  Google Scholar 

  7. Prasad, K. N. 1973. Role of cyclic AMP in differentiation of neuroblastoma cells in culture. In: J. Schultz, and H. G. Gratzner, (Eds.),The Role of Cyclic Nucleotides in Carcinogenesis Vol. 6. Academic Press, New York, pp. 207–247.

    Google Scholar 

  8. Rosenberg, R. N., L. Vandeventer, L. De Francesco, and M. K. 1971. Regulation of the synthesis of choline-0-acetyltransferase and thymydylate synthetase in mouse neuroblastoma in culture. Proc. Natl. Acad. Sci. U.S.A. 68: 1436–1440.

    Article  PubMed  CAS  Google Scholar 

  9. Bachrach, U. 1976. Induction of ornithine decarboxylase in glioma and neuroblastoma cells. FEBS Lett. 68: 63–67.

    Article  PubMed  CAS  Google Scholar 

  10. Prasad, K. N. and A. W. Hsie. 1971. Morphologic differentiation of mouse neuroblastoma cells induced in vitro by dibutyryl adenosine 3′:5′-cyclic monophosphate. Nature [New Biol.] 233: 141–142.

    CAS  Google Scholar 

  11. Prasad, K. N., B. Mandal, J. C. Waymire, G. J. Lees, A. Vernadakis, and N. Weiner. 1973. Basal level of neurotransmitter synthesizing enzymes and effect of cyclic AMP agents on the morphological differentiation of isolated neuroblastoma clones. Nature [New Biol.] 241: 117–119.

    CAS  Google Scholar 

  12. Sheppard, H., G. Wiggan, and W. H. Tsien. 1972. Structure-activity relationships for inhibitors of phosphodiesterase from erythrocytes and other tissue. In: P. Greengard, G. A. Robinson, and R. Paoletti (Eds.),Advances in Cyclic Nucleotide Research. Vol. 1. Raven Press, New York, pp. 102–112.

    Google Scholar 

  13. Prasad, K. N. 1975. Differentiation of neuroblastoma cells in culture. Biol. Rev. 50: 129–165.

    PubMed  CAS  Google Scholar 

  14. Eagle, H. 1974. Effect of environmental pH on the growth and function of culture mammalian cells. In: B. Clarkson, and R. Baserga (Eds.)Control of Proliferation in Animal Cells. Cold Spring Harbor Laboratory, Cold Spring Harbor, pp. 1–11.

    Google Scholar 

  15. O’Dea, R. F., M. K. Haddox, and N. D. Goldberg. 1971. Interaction with phosphodiesterase of free and kinase complexed cyclic adenosine 3′,5′-monophosphate. J. Biol. Chem. 246: 6183–6190.

    PubMed  CAS  Google Scholar 

  16. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with Folin phenol reagent. J. Biol. Chem. 193: 265–275.

    PubMed  CAS  Google Scholar 

  17. Kumar, S., G. Becker, and K. N. Prasad. 1975. Cyclic adenosine 3′,5′-monophosphate phosphodiesterase activity in malignant and cyclic adenosine 3′,5′-monophosphate-induced “differentiated” neuroblastoma cells. Cancer Res. 35: 82–87.

    PubMed  CAS  Google Scholar 

  18. Cheung, W. Y. 1970. Cyclic nucleotide phosphodiesterase. In: P. Greengard, and E. Costa (Eds.),Role of Cyclic AMP in Cell Function. Raven Press, New York, pp. 51–65.

    Google Scholar 

  19. Prasad, K. N., G. Becker, and K. Tripathy. 1975. Differences and similarities between guanosine 3′,5′-cyclic monophosphate phosphodiesterase and adenosine 3′,5′-cyclic monophosphate phosphodiesterase activities in neuroblastoma cells in culture. Proc. Soc. Exp. Biol. Med. 149: 757–762.

    PubMed  CAS  Google Scholar 

  20. Bourne, H. R., G. M. Tomkins, and S. Dion. 1973. Regulation of phosphodiesterase synthesis: requirement for cyclic adenosine monophosphate-dependent protein kinase. Science 181: 952–954.

    Article  PubMed  CAS  Google Scholar 

  21. Prasad, K. N., D. Fogleman, M. Gaschler, P. K. Sinha, and J. L. Brown. 1976. Cyclic nucleotide-dependent protein kinase activity in malignant and cyclic AMP-induced “differentiated” neuroblastoma cells in culture. Biochem. Biophys. Res. Commun. 68: 1248–1255.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Radiology, University of Colorado Medical Center, 80262, Denver, Colorado

    Pramod K. Sinha & Kedar N. Prasad

Authors
  1. Pramod K. Sinha
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kedar N. Prasad
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by USPHS NS-09230, and DRG-1273 from Damon Runyon-Walter Winchell Cancer Fund.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sinha, P.K., Prasad, K.N. A further study on the regulation of cyclic nucleotide phosphodiesterase activity in neuroblastoma cells: Effect of growth. In Vitro 13, 497–501 (1977). https://doi.org/10.1007/BF02615142

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02615142

Key words

Search

Navigation