Abstract
Random spore analysis of crosses between a strain bearing theade6-M26 hotspot mutation and strains bearing otherade6 mutations was performed. Recombinant prototroph frequencies increase with increasing distance fromM26 for mutations both 5′ and 3′ ofM26. Maximum prototroph frequencies are obtained for mutations lying more than 700 nucleotides down-stream fromM26. Similar results are obtained for crosses with theade6-M375 control mutation, but the prototroph frequencies are lower. The factor of stimulation of recombination byM26 as compared to theM375 control (M26 marker effect) also displays distance-dependence. These results are discussed in the context of the mechanism ofM26 recombination, as well as in relation to recombination initiation, hybrid DNA formation, and mismatch repair atade6.
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Zahn-Zabal, M., Kohli, J. The distance-dependence of the fission yeastade6-M26 marker effect in two-factor crosses. Curr Genet 29, 530–536 (1996). https://doi.org/10.1007/BF02426957
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DOI: https://doi.org/10.1007/BF02426957