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Secretion ofTrichoderma reesei β-glucosidase bySaccharomyces cerevisiae

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Abstract

An intronless form of thebgl1 gene encoding an extracellularβ-glucosidase fromTrichoderma reesei was expressed in the yeast Saccharomyces cerevisiae under the control of the yeast GAL 1 promoter. Transformation of a yeast strain with this vector resulted in transformants that produce and secrete activeβ-glucosidase into the growth medium. Additionally, active recombinantβ-glucosidase protein was shown to be localized predominantly in the periplasmic space by using ap-nitrophenylβ-D-glycoside hydrolysis assay against fractionated yeast cells. The apparent size of the recombinant enzyme was 10–15 kDa larger than that of the native form. Treatment of the recombinantβ-glucosidase with endoglycosidase-H indicated the apparent increase in size was due to N-linked glycosylation.

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  1. Genencor International, 180 Kimball Way, 94080, South San Francisco, CA, USA

    C. Cummings & T. Fowler

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  1. C. Cummings
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  2. T. Fowler
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Communicated by M. S. Esposito

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Cummings, C., Fowler, T. Secretion ofTrichoderma reesei β-glucosidase bySaccharomyces cerevisiae . Curr Genet 29, 227–233 (1996). https://doi.org/10.1007/BF02221552

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  • DOI: https://doi.org/10.1007/BF02221552

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