Abstract
Adding 2–30 μM jasmonic acid (JA) to photomixotrophic suspension cultures of soybean increased the level of several soluble polypeptides isolated by SDS-PAGE. The major polypeptides affected by JA treatment were at Mr 31,200 (p31) and Mr 39,000. Spraying leaves of soybean seedlings with 10–50 μM JA also increased the level of several soluble polypeptides including p31. The use of Con A affinity chromatography demonstrated that p31 was a glycoprotein and that JA increased the level of three other glycoproteins at Mr 22,000, 33,000, and 52,000. The JA treatment did not alter the growth or morphology of the seedlings. JA at 2–30 μM did not significantly inhibit the growth of the cultured cells and did not significantly alter the chlorophyll concentration. However, JA at concentrations above 30 μM inhibited growth and chlorophyll levels in cultured cells. The suspension cultured cells could provide a reliable bioassay for jasmonic acid.
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This paper represents cooperative investigations of the U.S. Department of Agriculture, Agricultural Research Service, and the North Carolina Agricultural Research Service, Raleigh, North Carolina 27695-7601. Paper No. 10997 of the journal series of the North Carolina Agricultural Research Service, Raleigh, NC 27695-7601.
Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture or the North Carolina Agricultural Research Service and does not imply its approval to the exclusion of other products that may also be suitable.
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Anderson, J.M. Jasmonic acid-dependent increases in the level of specific polypeptides in soybean suspension cultures and seedlings. J Plant Growth Regul 7, 203–211 (1988). https://doi.org/10.1007/BF02025263
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DOI: https://doi.org/10.1007/BF02025263