Summary
The effect of substrate concentration (S 0) on the fermentation parameters of a sugar mixture byPichia stipitis Y 7124 was investigated under anaerobic and microaerobic conditions. Under microaerobiosisP. stipitis maintained high ethanol yield and productivity when initial substrate concentration did not exceed 150 g/l; ethanol yield of about 0.40 g/g and volumetric productivity up to 0.39 g/l per hour were obtained. Optimal specific ethanol productivity (0.2 g/g per hour) was observed withS 0=110 g/l. Under anaerobic conditionsP. stipitis exhibited the highest fermentative performances atS 0=20 g/l; it produced ethanol with a yield of 0.42 g/g, with a specific rate of 1.1 g/g per day. When the initial substrate level increased, specific ethanol productivity declined gradually and ethanol yield was dependent on the degree of utilization of each sugar in the mixture.
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Abbreviations
- E m :
-
maximum produced ethanol (g/l)
- E 0 :
-
initial ethanol (g/l)
- E v :
-
evaporated ethanol (g/l)
- Q p :
-
volumetric productivity of ethanol (g ethanol/l per hour or g/l per day)
- q p :
-
specific productivity of ethanol (g ethanol/g cells per hour)
- q pm :
-
maximum specific productivity of ethanol (g/l per hour)
- S 0 :
-
initial substrate concentration (g/l)
- t f :
-
time at which produced ethanol is maximum (h)
- Y p/s :
-
ethanol yield (g ethanol produced/g substrate utilized)
- Y x/s :
-
cell yeild (g cells produced/g substrate utilized)
- Y xo/xy :
-
xylitol yield (g xylitol produced/g xylose utilized)
- α:
-
probability coefficient
- μ:
-
specific growth rate coefficient (h-1 or d-1)
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Delgenes, J.P., Moletta, R. & Navarro, J.M. Fermentation ofd-xylose,d-glucose andl-arabinose mixture byPichia stipitis Y 7124: sugar tolerance. Appl Microbiol Biotechnol 29, 155–161 (1988). https://doi.org/10.1007/BF01982895
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DOI: https://doi.org/10.1007/BF01982895