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Differential response of human basophils and mast cells to recombinant chemokines

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Abstract

Chemokines are proinflammatory peptides regulating the functions of various hematopoietic cells. We have analyzed the effects of seven recombinant human (rh) chemokines (MCAF, RANTES, MIP-1α, MIP-1β, IL-8, GRO, and IP-10) on the growth and function of human basophils and mast cells. We found that MCAF, but not RANTES, MlP-la, MIP-1β, IL-8, GRO, or IP-10, causes direct and dose-dependent histamine release from basophils (MCAF, 5 μg/ml: 26.9 ± 3.4%; other chemokines: < 5% of total histamine). An increased (2.1 to 3.5-fold) response to MCAF was obtained when basophils were preincubated with rh interleukin-3 (100 units/ml). Moreover, IL-3-primed basophils became responsive to physiologic concentrations (< 1 μg/ml) of MCAF, IL-8, and RANTES. None of the chemokines tested was able to induce histamine secretion in mast cells obtained from lung (n=2), skin (n=1), uterus (n=3), or tonsils (n=3), even when cells had been preincubated with the mast cell agonist SCF. The chemokines also failed to modulate the expression of activation antigens (CD11b/C3biR, CD25/IL-2Rβ, CD63, IL-3Rα, CD117/c-kit) on the mast cell line HMC-1 or the basophil cell line KU-812 and were unable to induce differentiation of basophils or mast cells in culture. Together, our results show that basophils respond to rhIL-8, rhMCAF, and rhRANTES and that, unlike human basophils, human mast cells are unresponsive to recombinant chemokines.

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Authors and Affiliations

  1. Department of Internal Medicine I, Division of Hematology and Hemostaseology, University of Vienna, Währinger Gürtel 18–20, A-1090, Vienna, Austria

    W. Füreder, H. Agis, H. Semper, F. Keil, K. Lechner & P. Valent

  2. Department of Urology, University of Vienna, Austria

    U. Maier

  3. Department of Surgery, University of Vienna, Austria

    M. R. Müller

  4. Department of Gynecology and Obstetrics, University of Vienna, Austria

    K. Czerwenka

  5. Department of Otolaryngology, Hospital Barmherzige Brüder, Vienna, Austria

    H. Höfler

Authors
  1. W. Füreder
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  2. H. Agis
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  3. H. Semper
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  4. F. Keil
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  5. U. Maier
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  6. M. R. Müller
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  7. K. Czerwenka
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  8. H. Höfler
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  9. K. Lechner
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  10. P. Valent
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Additional information

This work was supported by FWF Austria, grant P-9359, and by Sandoz, Vienna

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Füreder, W., Agis, H., Semper, H. et al. Differential response of human basophils and mast cells to recombinant chemokines. Ann Hematol 70, 251–258 (1995). https://doi.org/10.1007/BF01784044

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  • DOI: https://doi.org/10.1007/BF01784044

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