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Hydrophobic surface properties of erythrocytes studied by affinity partition in aqueous two-phase systems

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Summary

Erythrocytes from various species have been partitioned in aqueous two-phase systems consisting of water, dextran, poly-(ethylene glycol), salt and buffer. The terminal hydroxyl groups of the latter polymer were esterified with palmitic, oleic, linoleic and linolenic acids, as well as with deoxycholic acid. In a two-phase system containing unesterified poly(ethylene glycol) the erythrocytes are exclusively in the dextran-rich lower phase. When the poly(ethylene glycol) is esterified the red blood cells collect at the interface and/or in the poly(ethylene glycol)-rich upper phase depending on the type and concentration of esterified acid. Palmitate ester is most effective in increasing the affinity of the cells for the upper phase, followed by oleate, linolate, linolenate, and deoxycholate esters. The partition behaviour of erythrocytes from various species differs considerably. Two groups can be distinguished: one consisting of erythrocytes from dog, guinea pig and rat, the other from human, sheep and rabbit. This division can be correlated to the content of sphingomyelin and phosphatidyl choline in the erythrocyte membranes.

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Author notes

  1. Per-Åke Albertsson

    Present address: Department of Biochemistry, University of Lund, S-220 07, LUND, Sweden

Authors and Affiliations

  1. Department of Biochemistry, University of Umeå, S-901 87, Umeå, Sweden

    Eva Eriksson, Per-Åke Albertsson & Göte Johansson

Authors
  1. Eva Eriksson
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  2. Per-Åke Albertsson
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  3. Göte Johansson
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Eriksson, E., Albertsson, PÅ. & Johansson, G. Hydrophobic surface properties of erythrocytes studied by affinity partition in aqueous two-phase systems. Mol Cell Biochem 10, 123–128 (1976). https://doi.org/10.1007/BF01742204

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  • DOI: https://doi.org/10.1007/BF01742204

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