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Effects of antineoplastic phospholipids on parameters of cell differentiation in U937 cells

  • Original Papers
  • Experimental Oncology
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Summary

The proliferation of the human promonocytic leukemia cell line U937 is inhibited by several ether lipids, ether lipid analogues and by phorbol esters. An early effect of this retardation of cell growth is the induction of a basic chromosomal protein, histone H10. Northern blot analysis of H10 mRNA levels reveals an increase of the mRNA concentration within a few hours after addition of hexadecylphosphocholine and 1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine. This early effect on the synthesis of a subtype of H1 proteins precedes the expression of several parameters of the monocytic differentiation of U937 cells.

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Abbreviations

ES-16-OH:

1-O-palmitoyl-sn-glycero-3-phosphocholine

ET-18-OCH3 :

1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine

He-PC:

hexadecylphosphocholine

TPA:

12-O-tetraclecanoylphorbol 13-acetate

He-PC6 :

hexadecylphospho(N,N,N-trimethylamino)-hexanol

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Authors and Affiliations

  1. Abteilung Molekularbiologie, Institut für Biochemie, D-3400, Göttingen, Federal Republic of Germany

    Christa Hochhuth & Detlef Doenecke

  2. Abteilung Hämatologie/Onkologie, Universitätsklinikum, D-3400, Göttingen, Federal Republic of Germany

    Dinko Berkovic & Clemens Unger

  3. Max-Planck-Institut für Biophysikalische Chemie, D-3400, Göttingen, Federal Republic of Germany

    Hansjörg Eibl

Authors
  1. Christa Hochhuth
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  2. Dinko Berkovic
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  3. Hansjörg Eibl
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  4. Clemens Unger
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  5. Detlef Doenecke
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Dedicated to Professor Dr. D. Schmähl on the occasion of his 65th birthday

Supported by the Deutsche Forschungsgemeinschaft (D.D.) and the Ministry for Research and Technology (C.U.)

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Hochhuth, C., Berkovic, D., Eibl, H. et al. Effects of antineoplastic phospholipids on parameters of cell differentiation in U937 cells. J Cancer Res Clin Oncol 116, 459–466 (1990). https://doi.org/10.1007/BF01612994

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  • DOI: https://doi.org/10.1007/BF01612994

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