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Isolation of embryonic chick myosatellite and pluripotent stem cells

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Journal of tissue culture methods

Summary

The current study outlines the isolation and culture of two populations of cells derived from Day 11 embryonic chick leg muscle and associated connective tissues. The two populations consisted of myogenic lineage-committed stem cells (myosatellite stem cells) and lineage-uncommitted stem cells (pluripotent stem cells). After long-term culture the lineage-uncommitted stem cell population displayed differentiated phenotypes suggestive of the following adult tissues, fibroblasts, muscle, fat, cartilage, and bone.

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Young, H.E., Ceballos, E.M., Smith, J.C. et al. Isolation of embryonic chick myosatellite and pluripotent stem cells. Journal of Tissue Culture Methods 14, 85–92 (1992). https://doi.org/10.1007/BF01404749

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