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Upregulation of Fas-induced apoptosis by interferon-γ accompanied by increased ICE expression in renal cell cancer cells

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Abstract

The integration of Fas/Apo-1 (CD95) by Fas ligand or anti-Fas antibody induces apoptosis, and this system plays a pivotal role for the lysis of target cells by cytotoxic T lymphocytes. Fas-mediated apoptosis is also increased by a prior incubation of Fas-bearing cells with interferon(IFN)-γ. Interleukin-1-β converting enzyme (ICE) and/or CPP32, or other members of ICE family act as direct cell death executors downstream of this mechanism, and a tetrapeptide inhibitor of these cysteine proteases blocks Fas-mediated apoptosis. In this study, we examined the effect of IFN-γ on Fas-mediated apoptosis in ACHN cells. IFN-γ augmented apoptosis in a dose dependent manner and reached a plateau at 400 U/ml when exposed for 48 h before the end of culture. The kinetics revealed a significant increase in apoptosis after 24 h. Exposing ACHN cells to IFN-γ increased pro-ICE expression accompanied with a decrease of pro-CPP32. These results suggest that direct enhancement of ICE expression and/or upregulation of conversion of pro-CPP32 to active form increases Fas-mediated apoptosis by IFN-γ in ACHN cells.

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Authors and Affiliations

  1. Department of Urology, Niigata University School of Medicine, Asahimachi 1, 951, Niigata, Japan

    Y. Tomita, V. Bilim, M. Takeda & K. Takahashi

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  1. Y. Tomita
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  2. V. Bilim
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  3. M. Takeda
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  4. K. Takahashi
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Tomita, Y., Bilim, V., Takeda, M. et al. Upregulation of Fas-induced apoptosis by interferon-γ accompanied by increased ICE expression in renal cell cancer cells. Apoptosis 1, 209–212 (1996). https://doi.org/10.1007/BF01321104

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  • DOI: https://doi.org/10.1007/BF01321104

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