Abstract
Several integrin heterodimers such as α2β1, α6β4 and αvβ5 are expressed on basal keratinocytes of the epidermis. Because overexpression of these integrins as well as induction of the intercellular adhesion molecule-1 (ICAM-1) have been found in inflammatory dermatoses, we sought to determine whether these modulations could be used as markers of skin irritation. In normal epidermis, topical application of 1% sodium dodecyl sulphate (SDS) for 24 h caused an upregulation of α3, β1, α6, β4, αv, β5 and to a lesser extent α2 integrin chains as well as an induction of ICAM-1. To investigate whether these parameters could also be used for evaluation of skin irritancy in vitro, SDS was applied for 24 h to reconstructed epidermis on de-epidermized dermis (RE-DED). In RE-DED, integrin overexpression and aberrant α5 expression was seen under normal in vitro culture conditions and topical application of SDS caused only marginal additional upregulation. We could not detect any ICAM-1 reactivity on either normal or irritated RE-DED. Our results demonstrate that the modulation of integrin and ICAM-1 expression can be used as markers of irritation of the epidermis in vivo, but not in vitro.
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This work was supported in part by the Bundesministerium für Forschung und Technik, Bonn, Germany (BMFT: 421-4001-01HK9195).
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von den Driesch, P., Fartasch, M., Hüner, A. et al. Expression of integrin receptors and ICAM-1 on keratinocytes in vivo and in an in vitro reconstructed epidermis: Effect of sodium dodecyl sulphate. Arch Dermatol Res 287, 249–253 (1995). https://doi.org/10.1007/BF01105074
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DOI: https://doi.org/10.1007/BF01105074