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Technique of separation of germ layers in rat embryonic shields

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Summary

A microsurgical technique for the separation of germ layers in two- and three-layered rat embryonic shields is described.

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Abbreviations

A:

amnion

Ac:

amniotic cavity

All:

allantois

Ec:

definitive ectoderm

EEP:

extra-embryonic part

En:

definitive endoderm

EPc:

ectoplacental cone

ESh:

einbryonic shield

HF:

head fold

HN:

Hensen's node

Me:

definitive mesoderm

NG:

neural groove

pEc:

primitive ectoderm

pEn:

primitive endoderm

PS:

primitive streak

PS/2:

the cut edge obtained by median cutting through the primitive streak

Rm:

Reichert's membrane

I:

operating needle

II:

supporting (fixing) needle

References

  • Levak-Švajger, B., Švajger, A.: Differentiation of endodermal tissues in homografts of primitive ectoderm from two-layered rat embryonic shields. Experientia27, 683–684 (1971)

    PubMed  Google Scholar 

  • Levak-Švajger, B., Švajger, A.: Investigation on the origin of the definitive endoderm in the rat embryo. J. Embryol. exp. Morph.32, 445–459 (1974)

    PubMed  Google Scholar 

  • Levak-Švajger, B., Švajger, A., Škreb, N.: Separation of germ layers in presomite rat embryos. Experientia25, 1311–1312 (1969)

    Google Scholar 

  • Moscona, A., Trowell, O. A., Willmer, E. N.: Methods. In Cells and Tissues in Culture (ed. E. N. Willmer), vol. I, London-New York: Academic Press (1965)

    Google Scholar 

  • New, D. A. T.: The Culture of Vertebrate Embryos. London: Logos Press (1966)

    Google Scholar 

  • Nicholas, J. S.: Experimental methods and rat embryos. In: The Rat in Laboratory Investigation (ed. E. J. Parris and J. A. Griffith). New York: Hafner (1962)

    Google Scholar 

  • Szabó, G.: A modification of the technique of “skin splitting” with trypsin. J. Path. Bact.70, 545 (1955)

    PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Institute of Histology and Embryology Faculty of Medicine, University of Zagreb, P.O. Box 166, 41001, Zagreb, Yugoslavia

    A. Švajger & B. Levak-Švajger

  2. Institute of Biology Faculty of Medicine, University of Zagreb, P.O. Box 166, 41001, Zagreb, Yugoslavia

    A. Švajger & B. Levak-Švajger

Authors
  1. A. Švajger
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  2. B. Levak-Švajger
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Švajger, A., Levak-Švajger, B. Technique of separation of germ layers in rat embryonic shields. Wilhelm Roux' Archiv 178, 303–308 (1975). https://doi.org/10.1007/BF00848065

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  • DOI: https://doi.org/10.1007/BF00848065

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