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Analysis of the mitochondrial and nuclear genomes of two basidiomycetes, Coprinus cinereus and Coprinus stercorarius

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Summary

The mitochondrial and nuclear genomes of Coprinus stercorarius and C. cinereus were compared to assess their evolutionary relatedness and to characterize at the molecular level changes that have occurred since they diverged from a common ancestor. The mitochondrial genome of C. stercorarius (91.1 kb) is approximately twice as large as that of C. cinereus (43.3 kb). The pattern of restriction enzyme recognition sites shows both genomes to be circular, but reveals no clear homologies; furthermore, the order of structural genes is different in each species. The C. stercorarius mitochondrial genome contains a region homologous to a probe derived from the yeast mitochondrial var1 gene, whereas its nuclear genome does not. By contrast, the C. cinereus nuclear, but not mitochondrial, genome contains a region homologous to the var1 probe. Only a small fraction of either the nuclear or mitochondrial genomes, perhaps corresponding to the coding sequences, is capable of forming duplexes in interspecies solution reassociations, as measured by binding to hydroxylapatite. Those sequences capable of reassociating were found to have approximately 15% divergence for the mitochondrial genomes and 7%–15% divergence for the nuclear genomes, depending on the conditions of reassociation.

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Author notes

  1. Christine A. Weber

    Present address: Biomedical Sciences Division, L-452, Lawrence Livermore National Laboratory, University of California, 94550, Livermore, CA, USA

  2. Michael E. S. Hudspeth

    Present address: Plant Molecular Biology Center, Department of Biological Sciences, Northern Illinois University, 60115-2861, DeKalb, IL, USA

  3. Gordon P. Moore

    Present address: Gene Expression Group, NEN Products Division, DuPont BRL-500-3, 01862, North Billerica, MA, USA

  4. Lawrence I. Grossman

    Present address: Science, 1333 H Street, N. W., 20005, Washington, DC, USA

Authors and Affiliations

  1. Department of Cellular and Molecular Biology, Division of Biological Sciences, The University of Michigan, 48109, Ann Arbor, MI, USA

    Christine A. Weber, Michael E. S. Hudspeth, Gordon P. Moore & Lawrence I. Grossman

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  1. Christine A. Weber
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  2. Michael E. S. Hudspeth
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  3. Gordon P. Moore
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  4. Lawrence I. Grossman
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Weber, C.A., Hudspeth, M.E.S., Moore, G.P. et al. Analysis of the mitochondrial and nuclear genomes of two basidiomycetes, Coprinus cinereus and Coprinus stercorarius . Curr Genet 10, 515–525 (1986). https://doi.org/10.1007/BF00447385

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  • DOI: https://doi.org/10.1007/BF00447385

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