Summary
The expression of the lacZ gene of E. coli in S. cerevisiae has been studied. The enzymatic activity coded by the lacZ gene in E. coli, β-galactosidase, is detectable in yeast cells harboring a chimeric plasmid carrying the gene. On the basis of size and immunological criteria, no difference was detected between the Coli-in-yeast β-galactosidase and the E. coli enzyme.
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Panthier, JJ., Fournier, P., Heslot, H. et al. Cloned ß-galactosidase gene of Escherichia coli is expressed in the yeast Saccharomyces cerevisiae . Curr Genet 2, 109–113 (1980). https://doi.org/10.1007/BF00420622
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DOI: https://doi.org/10.1007/BF00420622