Summary
In order to study resistance to DNA damaging agents, yeast DNA segments conferring hyperresistance in this organism to such genotoxic agents were selected for among yeast cells transformed by a yeast genome library based on the multi-copy vector plasmid YEp13. Genetic variants hyperresistant to 4-nitroquinohne-N-oxide, formaldehyde, and alkylating agents were isolated and the respective hyperresistance determinants shown to co-segregate with the vector plasmid. Phenotypical characterization indicated different degrees of resistance, few cases of cross-resistance and differing structural stability of the cloned DNA. By transfer to E. coli and subsequent retransformation of yeast a number of plasmids was shown to stably carry the genetic information for hyperresistance.
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Dedicated to Prof. Dr. Fritz Kaudewitz on the occasion of his 65th birthday
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Ruhland, A., Brendel, M. & Haynes, R.H. Hyperresistance to DNA damaging agents in yeast. Curr Genet 11, 211–215 (1986). https://doi.org/10.1007/BF00420609
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DOI: https://doi.org/10.1007/BF00420609