Abstract
Tritiated 8-methoxypsoralen (8-MOP), with radioactivity in the ring system, was injected into both pigmented rabbits and albino rabbits; at 0.5, 1, 2, 4, 6, and 24 h after 8-MOP injection the animals' plasma and ocular radioactivities were determined by liquid scintillation counting. The experiment was done without photostimulation but in incomplete darkness.
The plasma radioactivity declined rapidly during the first hour, peak radioactivity occurring in the first samples of vascularized ocular structures taken 0.5 h after the injection. The radioactivity of these tissues generally declined rapidly and similarly in both animal groups. The 1, 2, and 4 h samples of pigmented animals formed an exception; in these the radioactivities in the iris-ciliary body and the retina-choroid of the pigmented animals showed increased activities that were statistically significant, and the plasma radioactivity was exceeded by two to three times.
The radioactivities in the internal parts of the eye were lower than in the vascular parts. The radioactivity in the lens reached a peak in the specimens taken 1–2 h after injection and remained at the level of the plasma radioactivity for the rest of the 24 h.
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References
Atlasik B, Stepien K, Wilczok T (1980) Interaction of drugs with ocular melanin in vitro. Exp Eye Res 30:325–331
Busch U, Koss FW, Schmid J (1979) Study of distribution with 8-Methoxypsoralen (8-MOP) in different tissues of rat. Arch Derm Res 264:125–126
Busch U, Schmid J, Koss FW, Zipp H, Zimmer A (1978) Pharmacokinetics and metabolite-pattern of 8-Methoxypsoralen in man following oral administration as compared to the pharmacokinetics in rat and dog. Arch Dermatol Res 262:255–265
Bäck O, Hollström E, Lidén S, Thorburn W (1980) Absence of cataract ten years after treatment with 8-Methoxypsoralen. Acta Dermatovener (Stockholm) 60:79–80
Ehrsson H, Eksborg S, Wallin J (1978) Metabolism of 8-Methoxypsoralen in man: Identification and quantification of 8-Hydroxypsoralen. Eur J Drug Metab Pharmacokin 3:125–128
El-Mofty AM, El-Mofty A (1979) Retrospective ocular study of patients receiving oral 8-Methoxypsoralen and solar irradiation for the treatment of vitiligo. Ann Ophthalmol 11:946–948
Glew WB (1979) Determination of 8-Methoxypsoralen in serum, aqueous, and lens: Relation to long-wave ultraviolet phototoxicity in experimental and clinical photochemotherapy. Tr Am Ophth Soc 77:464–514
Lerman S, Borkman RF (1977) A method for detecting 8-Methoxypsoralen in the ocular lens. Science 197:1287–1288
Lerman S, Jocoy M, Borkman RF (1977) Photosensitization of the lens by 8-Methoxypsoralen. Invest Ophthal Visual Sci 16:1065–1068
Lerman S, Megaw JM, Gardner K, Takei Y, Willis I (1981) Localization of 8-Methoxypsoralen in ocular tissues. Ophthal Res 13:106–116
Lerman S, Megaw J, Willis I (1980) Potential ocular complications from Puva therapy and their prevention. J Invest Dermatol 74:197–199
Ljunggren B, Carter DM, Albert J, Reid T (1980) Plasma levels of 8-Methoxypsoralen determined by high-pressure liquid chromatography in psoriatic patients ingesting drug from two manufacturers. J Invest Dermatol 74:59–62
Potts AM (1964) Further studies concerning the accumulation of polycyclic compounds on uveal melanin. Invest Ophthalmol 3:399–404
Salminen L (1978) Cloxacillin distribution in the rabbit eye after intravenous injection. Acta Ophthalmol 56:11–19
Smyth RD, Van Harken DR, Pfeffer M, Nardella PA, Vasiljev M, Pinto JS, Hottendorf GH (1980) Biological disposition of 8-Methoxalen in rat and man. Arzneim Forsch 30:1725–1730
Wulf HC, Hart J (1978) Accumulation of 8-Methoxypsoralen in the rat retina. Acta Ophthalmol 56:284–290
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Salminen, L., Wilén, G. Ocular kinetics of 8-methoxypsoralen. Albrecht von Graefes Arch. Klin. Ophthalmol. 217, 235–243 (1981). https://doi.org/10.1007/BF00411154
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DOI: https://doi.org/10.1007/BF00411154