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Fluxes and compartmentation of K+, Na+ and Cl−, and action of auxins in suspension-cultured Petroselinum cells

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Abstract

Transport of 86Rb+/K+, 22Na+, 36Cl−, and [3H]indole acetic acid (IAA) has been studied on suspension-cultured cells of the parsley, Petroselinum crispum (Mill) Nym. By compartmental analysis two intracellular compartments of K+, Na+, and Cl− have been identified and ascribed to the cytoplasm and vacuole; half-times of exchange were around 200 s and 5 h, respectively. According to the Ussing-Teorell flux equation, active transport is required for the influx into the cytoplasm at the plasmalemma (K+, Cl−) and the tonoplast (K+, Na+, Cl−). The plasmalemma permeability pattern, PK:PNa:PCl=1.00:0.24:0.38, features an increased chloride permeability compared with cells from higher plant tissues. IAA uptake showed an exponential timecourse, was half-maximal after 10 min, and a linear function of the IAA concentration from 10−9 to 10−5 M. IAA and 2,4-dichlorophenoxy acetic acid reduce the apparent influx of K+, Na+, Cl− during the initial 30 min after addition and subsequently accelerate both in- and efflux of these ions. We discuss that auxins could affect the ion fluxes in a complex way, e.g. by protonophorous activity and by control of the hypothetical proton pump.

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Abbreviations

IAA:

indole acetic acid

2,4-D:

dichlorophenoxy acetic acid

ϕ:

unidirectional transmembrane flux (molm−2 s−1)

ψ:

electrical potential difference (mV)

subscripts: c :

cytoplasmic

o :

outside

o :

vacuolar

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Authors and Affiliations

  1. Abteilung für Biophysik der Pflanzen, Institut für Biologie I der Universität, Auf der Morgenstelle 1, D-7400, Tübingen, Federal Republic of Germany

    Helmut Pfrüner & Friedrich-W. Bentrup

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  1. Helmut Pfrüner
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  2. Friedrich-W. Bentrup
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Dedicated to Professor Noe Higinbotham on the occasion of his 65th birthday

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Pfrüner, H., Bentrup, FW. Fluxes and compartmentation of K+, Na+ and Cl−, and action of auxins in suspension-cultured Petroselinum cells. Planta 143, 213–223 (1978). https://doi.org/10.1007/BF00387792

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  • DOI: https://doi.org/10.1007/BF00387792

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