Summary
With the help of a cDNA probe for a chalcone synthase gene of Petroselinum a cDNA clone for a chalcone synthase gene of Petunia hybrida could be identified. The homologous cDNA allowed the cloning of two genomic EcoRI fragments from Petunia hybrida containing complete chalcone synthase genes. It could be demonstrated that the genes on the two fragments are different and are not allelic but members of a gene family. The two genes are found in a variety of different Petunia lines including in the two conditional mutants affected in chalcone synthase expression in floral buds, White Joy and Red Star. The structure of the two chs genes from Petunia is compared to the chs gene from Antirrhinum majus.
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Berry-Lowe SL, McKnight TD, Shah DM, Meagher RB (1984) The nucleotide sequence, expression and evolution of one member of a multigene family encoding the small subunit of ribulose-1,5-bisphosphate carboxylase in soybean. J Mol Appl Gen 1:483–498
Boyer HW, Roulland-Dussoix (1969) A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol 41:459–472
Bonas U, Sommer H, Saedler H (1984) The 17 kb Tam-1 element of Antirrhinum majus induces a 3 bp duplication upon integration into the chalcone synthase gene. EMBO J 3:1015–1019
Buell GN, Wickens MP, Payvar F, Schimke RT (1978) Synthesis of full length cDNAs from four partially purified oviduct mRNAs. J Biol Chem 253:2471–2482
Davis RW, Hyman RW (1971) A study in evolution: the DNA base sequence homology between coliphages T7 and T3. J Mol Biol 62:287–301
Davis RW, Simon M, Davidson N (1971) Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. Methods Enzymol 21:413–428
Dooner HK (1983) Coordinate genetic regulation of flavonoid biosynthetic enzymes in maize. Mol Gen Genet 189:136–141
Galau GA, Dure L (1981) Developmental biochemistry of cotton embryogenesis and germination: changing mRNA populations as shown by reciprocal heterologous cDNA-mRNA hybridization. Biochemistry 20:4169–4178
Gerlach WL, Pryor AJ, Dennis ES, Ferl RJ, Sachs MM, Peacock WJ (1982) cDNA cloning and induction of the alcohol dehydrogenase gene (ADH) of maize. Proc Natl Acad Sci USA 79:2981–2985
Goldberg RB, Hoschek G, Kamalay JC, Timberlake WE (1978) Sequence complexity of nuclear and polysomal RNA in leaves of the tobacco plant. Cell 14:123–131
Goldberg RB, Hoschek G, Tam SH, Ditta GS, Breidenbach RW (1981) Abundance diversity and regulation of mRNA sequence sets in soybean embryogenesis. Dev Biol 82:201–217
Gagen G, Rubenstein I (1981) Complex organisation of zein genes in maize. Gene 13:239–249
Hahlbrock K (1981) Flavonoids. In: Stumpf PK, Conn EE (eds) Biochemistry of plants, vol 7. Academic Press, New York, pp 425–456
Hahlbrock K, Grisebach H (1979) Enzymic controls in the biosynthesis of lignin and flavonoids. Annu Rev Plant Physiol 30:105–130
Heller W, Hahlbrock K (1980) Highly purified flavanone synthase from parsley catalyzes the formation of naringenine chalcone. Arch Biochem Biophys 200:617–619
Hohn B (1979) In vitro packaging of λ and cosmid DNA. Methods Enzymol 68:299–309
Hopp HE, Rasmussen SK, Brandt A (1983) Organisation and transcription of B1 hordein genes in high lysine mutants of barley. Carlsb Res Comm 48:201–216
Hyldig-Nielson J, Jensen E, Paludan K, Wiborg O, Garret R, Jorgensen P, Marcker K (1982) The primary structure of two hemoglobin genes from soybean. Nucleic Acids Res 10:689–701
Kaback DB, Angerer LM, Davidson N (1979) Improved methods for the formation and stabilization of R-loops. Nucleic Acids Res 7:2499–2517
Kiper M, Bartels D, Herzfeld F, Richter G (1979) The expression of a plant genome in hnRNA and mRNA. Nucleic Acids Res 6:1961–1978
Kreuzaler F (1974) Zur Biosynthese aromatischer Verbindungen. Ph D Thesis, Univ Freiburg i Br FRG
Kreuzaler F, Ragg H, Fautz E, Kuhn DN, Hahlbrock K (1983) UV induction of chalcone synthase mRNA in cell suspension cultures of Petroselinum hortense. Proc Natl Acad Sci USA 80:2591–2593
Kuckuck (1936) Über vier neue Serien multipler Allele bei Antirrhinum majus Z indukt Abstamm Vererbungsl 71:429–440
Lawton MA, Dixon RA, Hahlbrock K, Lamb CJ (1983) Elicitor induction of mRNA activity. Eur J Biochem 130:131–139
Leder P, Tiemeier D, Enquist L (1977) EK2 derivatives of bacteriophage useful in the cloning of DNA from higher organisms: The gtWES system. Science 196:175–177
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA
McCormick, Mauvais J, Fedoroff N (1982) Evidence that the two sucrose synthase genes in maize are related Mol Gen Genet 187:494–500
Maxam AM, Gilbert W (1980) Sequencing endlabelled DNA with base specific chemical cleavages. Methods Enzymol 65:499–560
Mol NJJ, Schram AW, de Vlaming P, Gerats AGM, Kreuzaler F, Hahlbrock K, Reif HJ, Veltkamp E (1983) Regulation of flavonoid gene expression in Petunia hybrida: description and partial characterization of a conditional mutant in chalcona synthase gene expression. Mol Gen Genet 192:424–429
Rafalski JA, Scheets K, Metzler M, Peterson DM, Hedgecoth C, Soll DG (1984) Developmentally regulated plant genes: the nucleotide sequence of a wheat gliadin genomic clone. EMBO J 3:1409–1415
Reimold U, Kröger M, Kreuzaler F, Hahlbrock K (1983) Coding and 3′ non-coding nucleotide sequence of chalcone synthase mRNA and assignment of amino acid sequence of the enzyme. EMBO J 2:1801–1805
Schuler MA, Doyle JJ, Beachy RN (1983) Nucleotide homologies between glycosylated seed storage proteins of Glycine max and Paseolus vulgaris Plant Mol Biol 2:119–127
Schwarz-Sommer Z, Gierl A, Klösgen RB, Wienand U, Peterson PA, Saedler H (1984) The Spm (En) transposable element controls the excision of a two kb DNA insert at the wx m-8 allele of Zea mays. EMBO J 3:1021–1028
Spribille R, Forkmann G (1982) Genetic control of chalcone synthase activity in flowers of Antirrhinum majus. Phytochemistry 21:2231–2234
Sutcliffe G (1979) The complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harbor Symp Quant Biol 43:77–90
Taylor BA, Powell (1982) Isolation of Plant DNA and RNA. Focus 4:4–6
Wienand U, Langridge P, Feix G (1981) Isolation and characterization of a genomic sequence of maize coding for a zein gene. Mol Gen Genet 182:440–444
Wienand U, Sommer H, Schwarz Z, Sheperd N, Saedler H, Kreuzaler F, Ragg H, Fautz E, Hahlbrock K, Harrison BJ, Peterson PA (1982) A general method to identify plant structural genes among genomic DNA clones using transposable element induced mutations. Mol Gen Genet 187:195–201
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Communicated by P. Starlinger
Dedicated to Professor Georg Melchers to celebrate his 50-year association with the journal
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Reif, H.J., Niesbach, U., Deumling, B. et al. Cloning and analysis of two genes for chalcone synthase from Petunia hybrida . Molec Gen Genet 199, 208–215 (1985). https://doi.org/10.1007/BF00330261
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DOI: https://doi.org/10.1007/BF00330261