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Cloning of an Aspergillus niger invertase gene by expression in Trichoderma reesei

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Abstract

The filamentous fungus Aspergillus niger produces two glycosylated forms of the sucrose-hydrolysing enzyme, invertase. In contrast, some Trichoderma species lack invertase and are unable to utilise sucrose as a sole carbon source. Using an A. niger genomic library constructed in a cosmid vector containing the ura5 gene of Podospora anserina as a selectable marker, and the T. reesei ura5- strain as a sucrose-minus recipient strain, an A. niger invertase gene (suc1) has been cloned by a sib selection procedure. PAGE and enzyme analysis confirmed that transformants had acquired invertase activity. The cloned gene contained DNA sequences which were complementary to the amino-acid sequences of tryptic peptides found in invertase purified from A. niger. The suc1 invertase gene can be used as a dominant selectable marker for the transformation of Trichoderma strains.

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Authors and Affiliations

  1. Laboratoire de Génétique, Université de Bordeaux II URA CNRS 542, Avenue des Facultés, F-33405, Talence Cedex, France

    Thierry Bergès, Christian Barreau & Lynn M. Boddy

  2. Microbial Biochemistry and Genetics Group, Department of Life Science, University of Nottingham, University Park, NG7 2RD, Nottingham, UK

    John F. Peberdy & Lynn M. Boddy

Authors
  1. Thierry Bergès
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  2. Christian Barreau
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  3. John F. Peberdy
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  4. Lynn M. Boddy
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Communicated by P. P. Slonimski

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Bergès, T., Barreau, C., Peberdy, J.F. et al. Cloning of an Aspergillus niger invertase gene by expression in Trichoderma reesei . Curr Genet 24, 53–59 (1993). https://doi.org/10.1007/BF00324665

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  • DOI: https://doi.org/10.1007/BF00324665

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