Summary
The primary structure of the 49 K subunit of the respiratory chain NADH:ubiquinone reductase (complex I) from Neurospora crassa was determined by sequencing cDNA, genomic DNA and the N-terminus of the mature protein. The sequence lengths correlate to a molecular mass of 54002 daltons for the preprotein and 49239 daltons for the mature protein. The presequence consists of 42 amino acids of typical composition for sequences which target nuclear-encoded proteins into mitochondria. The mature protein consists of 436 amino acids and shows 64% similarity to a 49 K subunit of bovine heart NADH:ubiquinone reductase and 33% to a predicted translation product of an open reading frame in the chloroplast DNAs of Marchantia polymorpha and Nicotiana tabacum. Evidence for an iron-sulfur cluster in the subunit is discussed.
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References
Anderson S, de Bruijn MHL, Coulson AR, Eperon IC, Sanger F, Young IG (1982) J Mol Biol 156:683–717
Attardi G, Schatz G (1988) Annu Rev Cell Biol 4:289–333
Ballance DJ (1986) Yeast 2:229–236
Burger G, Werner S (1985) J Mol Biol 186:231–242
Chomyn A, Mariottini P, Cleeter MWJ, Ragan CI, Matsuno-Yagi A, Hatefi Y, Doolittle RF, Attardi G (1985) Nature 314:592–597
Chomyn A, Tsai Lai SSA (1989) Curr Genet 16:117–125
Davis RW, Botstein D, Roth JR (1980) Advanced bacterial genetics: a manual for genetic engineering. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
de Vries H, Alzner-DeWeerd B, Breitenberger CA, Chang DD, de Jonge JC, RajBhandary UL (1986) EMBO J 5:779–785
Feinberg AP, Vogelstein B (1983) Anal Biochem 132:6–13
Fearnley IM, Runswick MJ, Walker JE (1989) EMBO J 8:665–672
Friedrich T, Hofhaus G, Ise W, Nehls U, Schmitz B, Weiss H (1989) Eur J Biochem 180:173–180
Frischauf AM, Lehrach H, Poustka A, Murray N (1983) J Mol Biol 170:827–842
Harnisch U, Weiss H, Sebald W (1985) Eur J Biochem 149:95–99
Hartl FU, Pfanner N, Nicholson DW, Neupert W (1989) Biochem Biophys Acta 988:1–45
Hawlitschek G, Schneider H, Schmidt B, Tropschug M, Hartl FU, Neupert W (1988) Cell 53:795–806
Huynh TV, Young RA, Davis RW (1985) In: Glover DM (ed) DNA cloning, vol 1: practical approach. IRL Press, Oxford, pp 49–78
Ise W, Haiker H, Weiss H (1985) EMBO J 4:2075–2080
Kreader CA, Heckman JE (1987) Nucleic Acids Res 15:9027–9042
Leonard K, Haiker H, Weiss H (1987) J Mol Biol 194:277–286
Maniatis T, Fritsch EF, Sambrok J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbour Laboratory. Cold Spring Harbor, New York
Matzudaira P (1987) J Biol Chem 262:1035–1038
Mead DA, Szczesna-Skorupa E, Kemper B (1986) Prot Engineering 1:67–74
Michel R, Wachter E, Sebald W (1979) FEBS Lett 101:373–376
Nelson MA, Macino G (1987) Mol Gen Genet 206:307–317
Nishikimi M, Hosokawa Y, Toda H, Suzuki H, Ozawa T (1988) Biochem Biophys Res Commun 157:914–920
Ohnishi M, Ragan CI, Hatefi Y (1985) J Biol Chem 260:2782–2788
Ohyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H (1986) Nature 322:571–574
Pilkington S, Walker JE (1989) Biochemistry 28:3257–3264
Rackwitz HR, Zehetner G, Frischauf AM, Lehrach H (1984) Gene 30:195–200
Ragan CI (1987) Curr Top Bioenerget 15:1–36
Römisch J, Tropschug M, Sebald W, Weiss H (1987) Eur J Biochem 164:111–115
Runswick MJ, Gennis RB, Fearnley IM, Walker JE (1989) Biochemistry 28:9452–9459
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Schechtman MG, Yanovsky C (1983) J Mol Appl Genet 2:83–99
Schulte U, Arretz M, Schneider H, Tropschug M, Wachter E, Neupert W, Weiss H (1989) Nature 339:147–149
Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) EMBO J 5:2043–2049
Specht CA, DiRusso CC, Novotny CP, Ullrich RC (1982) Anal Biochem 119:158–163
Tuschen G, Sackmann U, Nehls U, Haiker H, Buse G, Weiss H (1990) J Mol Biol
Viebrock A, Perz A, Sebald W (1982) EMBO J 1:565–571
Videira A, Tropschug M, Wachter E, Schneider H, Werner S (1990) J Biol Chem
Videira A, Tropschug M, Werner S (1990) Biochem Biophys Res Commun 166:280–285
von Bahr-Lindström H, Galante YM, Persson M, Jornvall H (1983) Eur J Biochem 134:145–150
von Heijne G (1986) EMBO J 5:1335–1342
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Preis, D., van der Pas, J.C., Nehls, U. et al. The 49 K subunit of NADH: ubiquinone reductase (complex I) from Neurospora crassa mitochondria: primary structure of the gene and the protein. Curr Genet 18, 59–64 (1990). https://doi.org/10.1007/BF00321116
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DOI: https://doi.org/10.1007/BF00321116