Summary
A HeLa cell cDNA library on a yeast expression vector was used to complement auxotrophic markers of Saccharomyces cerevisiae. Clones complementing the ade2-101 mutation harbor a 1.5 kb poly(A)+ tailed insert with a 425 amino acid open reading frame hybridizing with two human mRNAs of 1.5 kb and 3.1 kb. Its 5′ half is homologous to Bacillus subtilis SAICAR synthetase (E.C.6.3.2.6.) and its 3′ terminal half corresponds to the catalytic subunit of Escherichia coli and B. subtilis AIR carboxylase (E.C.4.1.1.21). In agreement with these homologies, pADE2H1 clones complement both ade1 and ade2 mutants of S. cerevisiae, as was also recently reported for a 3.1 kb cDNA isolated from human hepatocytes.
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Casadaban M, Cohen S (1980) J Mol Biol 138:179–207
Cavallini B, Huet J, Plassat JL, Sentenac A, Egly JM, Chambon P (1988) Nature 334:77–80
Chambers A, Stanway C, Kingsman AJ, Kingsman SM (1988) Nucleic Acids Res 16:8245–8260
De Serres F (1969) Mutation Res 8:43–50
Ebbole DJ, Zalkin H (1987) J Biol Chem 262:8274–8287
Fitzgerald M, Shenk T (1981) Cell 24:251–260
Guarente L (1988) Cell 52:303–305
Hartley JL, Donelson JE (1980) Nature 286:860–865
Hinnen A, Hicks JB, Fink GR (1978) Proc Natl Acad Sci USA 75:1929–1933
Henikoff S (1986) J Mol Biol 190:519–528
Lee MG, Nurse P (1987) Nature 327:31–35
Marck C (1988) Nucleic Acids Res 16:1829–1836
Metzger D, White DH, Chambon P (1988) Nature 334:31–36
Nurse P (1990) Nature 344:503–508
Patey CAH, Shaw G (1973) Biochem J 135:543–545
Pearson WR, Lipman DJ (1988) Proc Natl Acad Sci USA 85:2444–2448
Ponticelli AS, Sena EP, Smith GR (1988) Genetics 119:491–497
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. A laboratory manual 2d edition. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Schild D, Brake AJ, Kiefer MC, Young D, Barr PJ (1990) Proc Natl Acad Sci USA 87:2916–2920
Sherman F, Fink GR and Hicks JB (1986) Laboratory Course Manual for Methods in Yeast Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Sigal IS, Gibbs JB, D'Alonzo JS, Scolnick EM (1986) Proc Natl Acad Sci USA 83:4725–4729
Stotz A and Linder P (1990) Gene (In press)
Struhl K (1988) Nature 332:649–650
Szankasi P, Heyer WD, Schuchert P, Kohli J (1988) J Mol Biol 204:917–925
Tiedeman AE, Keyhani J, Kamholz J, Daum HA, Gots JS, Smith JM (1989) J Bacteriol 171:205–212
Woods RA, Bevan EA (1966) Heredity 21:121–130
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Minet, M., Lacroute, F. Cloning and sequencing of a human cDNA coding for a multifunctional polypeptide of the purine pathway by complementation of the ade2-101 mutant in Saccharomyces cerevisiae . Curr Genet 18, 287–291 (1990). https://doi.org/10.1007/BF00318209
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DOI: https://doi.org/10.1007/BF00318209