Abstract
Primary gene amplification, i.e., mutation from one gene copy to multiple gene copies per genome, is important in genomic evolution, as a means of producing anti-cancer drug resistance, and is associated with the progression of tumor malignancy. Primary amplification has not been studied in normal eukaryotic cells because amplifications are extremely rare in these cells. A system has been developed to phenotypically identify co-amplifications of the ADH4 and CUP1 genes of Saccharomyces cerevisiae and 21 independent spontaneous amplifications have been isolated.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aladjem MI, Koltin Y, Lavi S (1988) Mol Gen Genet 211:88–94
Anderson RP, Roth J (1977) Annu Rev Micro 31:473–505
Becker DM, Guarente L (1991) High-efficiency transformation of yeast by electroporation. In: Guthrie C, Fink G (eds) Guide to yeast genetics and molecular biology. Academic Press, New York, pp 182–187
Berns EMJJ, Klijn JGM, van Putten WLJ, van Staveren IL, Portengen H, Foekens JA (1992) Cancer Res 52:1107–1113
Bishop JM (1991) Cell 64:235–248
Boeke JD, Trueheart J, Natsoulis G, Fink GR (1987) Methods Enzymol 154:164–175
Botstein D, Carl Falco S, Stewart SE, Brennan M, Scherer S, Stinchcomb DT, Struhl K, Davis RW (1979) Gene 8:17–24
Bridges CB (1936) Science 83:210–211
Carle GF, Olson MV (1985) Proc Natl Acad Sci USA 82:3756–3760
Chu G, Vollrath D, Davis RW (1986) Science 234:1582–1585
Ciriacy M (1976) Mol Gen Genet 145:327–333
Ciriacy M (1979) Mol Gen Genet 176:427–431
Denis C (1984) Genetics 108:833–844
Denis CL, Young ET (1983) Mol Cell Biol 3:360–370
Dorsey M, Peterson C, Bray K, Paquin CE (1992) Genetics 132: 943–950
Fogel S, Welch JW (1982) Proc Natl Acad Sci USA 79:5342–5346
Hansche PE, Beres V, Lange P (1978) Genetics 88:673–687
Hightower RC, Santi DV (1989) Electrophoresis 10:283–290
Karin M, Najarian R, Haslinger A, Valenuela P, Welch J, Fogel S (1984) Proc Natl Acad Sci USA 81:337–341
Kimura M, Ohta T (1974) Proc Natl Acad Sci USA 71:2848–2852
Klessig DJ, Berry JO (1983) Plant Mol Biol Rep 1:12–18
Link AJ, Olson MV (1991) Genetics 127:681–698
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Paquin CE, Williamson VM (1986) Mol Cell Biol 6:70–79
Paquin CE, Dorsey M, Crable S, Sprinkel K, Sondej M, Williamson VM (1992) Genetics 130:263–271
Slamon DJ, Godolphin W, Jones LA, Holt JA, Wong SG, Keith DE, Levin WJ, Stuart SG, Udove J, Ullrich A, Press MF (1989) Science 244:707–712
Schimke RT (1984) Cell 37:705–713
Sherman F (1991) Getting started with yeast. In: Guthrie C, Fink G (eds) Guide to yeast genetics and molecular biology. Academic Press, New York, pp 3–21
Spector LM, Fogel S (1992) Curr Genet 21:309–318
Stark GR (1983) Cancer Surveys 5:1–23
Stark GR, Debatisse M, Giulotto E, Wahl GM (1989) Cell 57:901–908
Tlsty TD (1990) Proc Natl Acad Sci USA 87:3132–3136
Tlsty TD, Margolin H, Lum K (1989) Proc Natl Acad Sci USA 86:9441–9445
Tlsty TD, White A, Sanchez J (1992) Science 255:1425–1427
Walton J, Paquin C, Kaneko K, Williamson V (1986) Cell 46:857–863
Welch J, Maloney DH, Fogel S (1990) Mol Gen Genet 222:304–310
Whittaker SG, Rockmill BM, Blechl AE, Maloney DH, Resnick MA, Fogel S (1988) Mol Gen Genet 215:10–18
Williamson VM, Young ET, Ciriacy M (1981) Cell 23:605–614
Windle B, Draper BW, Yin Y, O'Gorman S, Wahl GM (1991) Genes Dev 5:160–174
Wright JA, Smith HS, Watt FM, Hancock MC, Hudson DL, Stark GR (1990) Proc Natl Acad Sci USA 87:1791–1795
Author information
Authors and Affiliations
Additional information
Communicated by R.J. Rothstein
Rights and permissions
About this article
Cite this article
Dorsey, M.J., Hoeh, P. & Paquin, C.E. Phenotypic identification of amplifications of the ADH4 and CUP1 genes of Saccharomyces cerevisiae . Curr Genet 23, 392–396 (1993). https://doi.org/10.1007/BF00312624
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00312624