Abstract
We have developed a touch-fluorescence method using preparations from the outer surface of elevated and ulcerative malignant melanoma lesions. This method allows the demonstration of the melanogenic activity of melanoma cells within 30 min and has made it possible to definitively diagnose a lesion as malignant melanoma at the initial examination. In 21 cases clinically diagnosed as typical melanoma, 17 were definitively diagnosed as melanoma from the touch-fluorescence microscopic findings. Of the four cases in which no fluorescent tumor cells were found, two proved not to be melanoma. There was some correlation between the morbid types of primary melanoma and the configuration of fluorescent melanoma cells, the main cellular configuration of superficial spreading melanoma being round whereas that of nodular melanoma and acral lentigenous melanoma was pleomorphic. Based on the above results, this method was concluded to be extremely reliable for the preoperative diagnosis of elevated and ulcerative lesions of malignant melanomas, which are sometimes difficult to diagnose.
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Morishima T, Ishikawa T, Endo M, Tsujiguchi Y (1978) Pagatoid malignant melanoma and lentigo maligna melanoma of toe: A study with the fluoresence method (Falck and Hillarp). Arch Dermatol Res 262:275–283
Morishima T, Tatsumi F, Fukada E, Watanabe M, Nagashima N, Hanawa S (1983) Studies on amelanotic melanoma with the fluorescence method (Falck and Hillarp) and biochemical analysis of 5-S-cysteinyldopa in the tissues. Arch Dermatol Res 275:329–333
Gown AM, Vogel AM, Hoak D, Gough F, McNutt MA (1986) Monoclonal antibodies specific for melanocytic tumors distinguish subpopulations of melanocytes. Am J Pathol 123:195–203
Vennegoor C, Hageman P, Van Nouhuijs H, Ruiter DJ, Calafat J, Ringens PJ, Rümke P (1988) A monoclonal antibody specific for melanocytic differentiation. Am J Pathol 130:179–192
Angeli S, Koelma IA, Jan Fleuren G, Van Steenis G (1988) Malignant melanoma in fine needle aspirates and effusions: An immunochemical study using monoclonal antibodies. Acta Cytol 32:707–712
Morishima T, Nagashima N, Hanawa S, Fukada E, Kanematsu S, Shibata A (1986) Quick diagnosis of malignant melanoma with the touch-fluorescence method during operation. Cancer 57:2037–2041
Morishima T, Kanematsu S, Fukada E, Hanawa S, Nagashima N (1985) Cytodiagnosis of malignant melanoma by the touchfluorescence method. J Dermatol 12:286–289
Agrup G, Björklund A, Falck B, Jacobsson S, Lindvall O, Rorsman H (1977) Fluorescence histochemical demonstration of dopa thioethers by condensation with gaseous formaldehyde. Histochemistry 52:179–186
Corrodi H, Jonsson G (1967) Fluorescence method for the histochemical demonstration of biogenic monoamines. J Histochem Cytochem 15:65–78
Shibata A, Suzuki K, Hara H, Hanawa S, Morishima T (1989) The trial of pre-operative diagnosis of malignant melanoma with fine needle aspiration fluorescence method (in Japanese). Skin Cancer 4:133–137
Kopf AW, Mintzis M, Bart S (1975) Diagnostic accuracy in malignant melanoma. Arch Dermatol 111:1291–1292
Yamada T, Itou U, Watanabe Y, Ohashi S (1972) Cytologic diagnosis of malignant melanoma. Acta Cytol 16:70–76
Woyke S, Domagala W, Czerniac B, Strokowaska M (1980) Fine needle aspiration cytology of malignant melanoma of the skin. Acta Cytol 24:529–538
Kline TS, Kannan V (1982) Aspiration biopsy cytology and melanoma. Am J Clin Pathol 77:597–601
Morishima T, Tatsumi F, Fukada E, Saito M, Fujita M, Nagashima N, Hanawa S (1983) Cysteinyldopa isomers and dopa in lesions and urine of Japanese patients with malignant melanoma. Arch Dermatol Res 275:76–79
Rorsman H, Agrup G, Hansson C, Rosengren AM, Rosengren E (1979) In: Klaus SN (ed) Detection of Pheomelanin, vol 4. Karger, Basel, pp 244–252
Prota G (1980) Recent advances in the chemistry of melanogenesis in mammals. J Invest Dermatol 75:122–127
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Morishima, T., Shibata, A., Fujita, H. et al. Preoperative diagnosis of malignant melanoma using the touch-fluorescence method. Surg Today 23, 580–586 (1993). https://doi.org/10.1007/BF00311904
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DOI: https://doi.org/10.1007/BF00311904