Abstract
Brassica napus L.(cv Topas) plants tolerant to chlorsulfuron (CS) were isolated after selection experiments utilizing microspores and haploid protoplasts. The first microspore-derived plant (M-37,) was CS tolerant, haploid and sterile. Normal plant morphology and fertility was restored after colchicine doubling. A CS tolerant plant was also selected from protoplasts (P-26) isolated from microspore-derived embryo tissue and grown on medium containing CS. P-26 was aneuploid, CS tolerant and had very low fertility. The two selected lines produced selfed progeny which were tolerant to from 10–100 times the CS levels of the corresponding Topas plants. Microspores and protoplasts derived from the selfed plants were also CS tolerant. The segregation pattern for CS tolerance from reciprocally crossed progeny of M-37 and Topas was consistent with a semi-dominant nuclear mode of inheritance. Biochemical analysis of the two mutants indicated that the microspore-derived mutant and F1 crosses contained an altered acetohydroxyacid synthase (AHAS) enzyme, while the AHAS activity of the protoplast mutant was similar to Topas. Selfed seed from the M-37 plants have provided tolerance to CS in both greenhouse and field tests. S1 plants from a second microspore selected mutant (M-42) have tolerated 30 g/ha of CS in greenhouse tests. The two single-celled selection systems are discussed and the microspore selection system highlighted as a new method for in vitro selection.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barsby TL, Yarrow SA, Shepard JF (1986) Plant Cell Reports 5:101–103
Cardy B (1986) PhD Thesis, Univ. of Guelph, Guelph, Ontario, Canada
Chaleff RS, Ray TB (1984) Science 223: 1148–1151
Choung PV, Beversdorf WD (1985) Plant Science 39:219–226
Choung PV, Pauls KP, Beversdorf WD (1986) Plant Cell Reports 4:4–6
D'Amato F (1984) Critical Rev in Plant Science 3:73–112
Falco SC, Dumas KS (1985) Genetics 109:21–35
Gamborg OL, Miller RA, Ojima L (1968) Exp Cell Res 50:151–158
Glimelius K (1984) Physiol Plant 61:38–44
Haughn GW, Sommerville C (1986) Mol Gen Genet 204:430–434
Gressel J (1978) Outlook on Agriculture 9 (6):283–287
Gressel J (1985) Weed Physiology 2:159–189
Larkin PJ, Scowcroft WR (1981) Theor Appl Genet 60:197–214
Lichter R (1985) in H. Sorensen (Ed) Advances in the production and utilization of Cruciferous crops Martinus Mijhoff/Dr W Junk Pub: 268–277
Meins F (1983) Ann Rev Plant Physiol 34:327–346
Murishige T, Skoog F, (1962) Physiol Plant 15:473–497
Orton TJ, Browers MA (1985) Theor Appl Genet 69:637–643
Sebastian SA and Chaleff RS (1987) Crop Sci In Press
Shaffeek AP (1986) Agdex 606–1:Guide to crop protection in Alberta, Alberta Ariculture, Edmonton, Alberta, Canada
Smith AE, Hsiao AI (1985) Weed Science 33:555–557
Swanson EB, Coumans MP, Mu S-C, Barsby TL, Beversdorf WD (1987) Plant Cell Reports 6:94–97
Wondimagegnehu M, Foy CL (1985) Weed Science 33:564–568
Xu ZH, Davey MR, Cocking EC (1982) Plant Sci Lett 24:117–121
Yadav N, Mcdevitt RE, Benard S, Falco SC (1986) Proc Natl Acad Sci USA 83:4418–4422
Author information
Authors and Affiliations
Additional information
Communicated by F. Constabel
Rights and permissions
About this article
Cite this article
Swanson, E.B., Coumans, M.P., Brown, G.L. et al. The characterization of herbicide tolerant plants in Brassica napus L. after in vitro selection of microspores and protoplasts. Plant Cell Reports 7, 83–87 (1988). https://doi.org/10.1007/BF00270110
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00270110