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The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasodine in callus cultures of solanum laciniatum

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Abstract

In leaf derived callus cultures of Solanum laciniatum Ait. both phenolics and solasodine concentrations increased when medium phosphate or nitrogen concs. were reduced to one-eighth or when sucrose concentration was increased from 3 to 4–8 %. Under these conditions growth was reduced and final FW:DW fell. Growth was inhibited by sucrose depletion and nitrogen supple -mentation. On additional nitrogen the concentrations of phenolics and protein significantly increased, FW:DW was reduced and solasodine concentration was unaffected. In seedling derived cultures phosphate depletion resulted in a significant increase in phenolics concentration, an inhibition of growth and a rise in solasodine concentration.

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Author notes

  1. S. F. Chandler

    Present address: Department of Botany, University of Florida, 32611, Gainesville, Florida, USA

Authors and Affiliations

  1. Department of Plant Biology, University of Birmingham, B15 2TT, Birmingham, UK

    S. F. Chandler

  2. Centro de Investigaciòn y de Estudios Avanzados del I.P.N., Unidad Irapuato, Blvd. Diaz Ordaz 237 Desp. 101, 36660, Irapuato, Gt., Mexico

    J. H. Dodds

Authors
  1. S. F. Chandler
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  2. J. H. Dodds
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Communicated by J. K. Vasil

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Chandler, S.F., Dodds, J.H. The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasodine in callus cultures of solanum laciniatum . Plant Cell Reports 2, 205–208 (1983). https://doi.org/10.1007/BF00270105

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  • DOI: https://doi.org/10.1007/BF00270105

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